VvWRKY8 represses stilbene synthase genes through direct interaction with VvMYB14 to control resveratrol biosynthesis in grapevine

doi:10.1093/jxb/ery401

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	VvWRKY8 represses stilbene synthase genes through direct interaction with VvMYB14 to control resveratrol biosynthesis in grapevine
	Jiang, Jinzhu 1; Xi, Huifen 1; Dai, Zhanwu 4; Lecourieux, Fatma 4; Yuan, Ling 3; Liu, Xianju 1; Patra, Barunava 3; Wei, Yongzan ; Li, Shaohua ; Wang, Lijun
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2019
卷号	70 期号:2 页码:715-729
关键词	Fine-tuning grapevine protein interaction resveratrol VvMYB14 VvWRKY8
ISSN号	0022-0957
DOI	10.1093/jxb/ery401
文献子类	Article
英文摘要	Resveratrol (Res) is a stilbenoid, a group of plant phenolic metabolites derived from stilbene that possess activities against pests, pathogens, and abiotic stresses. Only a few species, including grapevine (Vitis), synthesize and accumulate Res. Although stilbene synthases (STSs) have been isolated and characterized in several species, the gene regulatory mechanisms underlying stilbene biosynthesis are still largely unknown. Here, we characterize a grapevine WRKY transcription factor, VvWRKY8, that regulates the Res biosynthetic pathway. Transient and stable overexpression of VvWRKY8 in grapevine results in decreased expression of VvSTS15/21 and VvMYB14, as well as in a reduction of Res accumulation. VvWRKY8 does not bind to or activate the promoters of VvMYB14 and VvSTS15/21; however, it physically interacts with VvMYB14 proteins through their N-terminal domains to prevent them from binding to the VvSTS15/21 promoter. Application of exogenous Res results in the stimulation of VvWRKY8 expression and in a decrease of VvMYB14 and VvSTS15/21 expression in grapevine suspension cells, and in the activation of the VvWRKY8 promoter in tobacco leaves. These results demonstrate that VvWRKY8 represses VvSTS15/21 expression and Res biosynthesis through interaction with VvMYB14. In this context, the VvMYB14-VvSTS15/21-Res-VvWRKY8 regulatory loop may be an important mechanism for the fine-tuning of Res biosynthesis in grapevine.
学科主题	Plant Sciences
电子版国际标准刊号	1460-2431
出版地	OXFORD
WOS关键词	WRKY TRANSCRIPTION FACTOR ; SALT STRESS TOLERANCE ; VITIS-VINIFERA ; ULTRAVIOLET C ; BOTRYTIS-CINEREA ; SALICYLIC-ACID ; ABSCISIC-ACID ; PROTEIN ; EXPRESSION ; REGULATOR
WOS研究方向	Plant Sciences
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000459350000028
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31171918, 31672120] ; Grape Breeding Project of Ningxia [NXNYYZ201502]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19670]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China 2.Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA 3.Univ Bordeaux, CNRS, INRA, EGFV,Bordeaux Sci Agro,ISVV, F-33140 Villenave Dornon, France 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Jinzhu,Xi, Huifen,Dai, Zhanwu,et al. VvWRKY8 represses stilbene synthase genes through direct interaction with VvMYB14 to control resveratrol biosynthesis in grapevine[J]. JOURNAL OF EXPERIMENTAL BOTANY,2019,70(2):715-729.
APA	Jiang, Jinzhu.,Xi, Huifen.,Dai, Zhanwu.,Lecourieux, Fatma.,Yuan, Ling.,...&Wang, Lijun.(2019).VvWRKY8 represses stilbene synthase genes through direct interaction with VvMYB14 to control resveratrol biosynthesis in grapevine.JOURNAL OF EXPERIMENTAL BOTANY,70(2),715-729.
MLA	Jiang, Jinzhu,et al."VvWRKY8 represses stilbene synthase genes through direct interaction with VvMYB14 to control resveratrol biosynthesis in grapevine".JOURNAL OF EXPERIMENTAL BOTANY 70.2(2019):715-729.