Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation

doi:10.1038/s41438-018-0083-5

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation
	Chai, Fengmei 3,4; Liu, Wenwen 3,4; Xiang, Yue 3; Meng, Xianbin 2; Sun, Xiaoming 4; Cheng, Cheng 3; Liu, Guotian 1; Duan, Lixin 6; Xin, Haiping ; Li, Shaohua 4
刊名	HORTICULTURE RESEARCH
	2019
卷号	6
ISSN号	2052-7276
DOI	10.1038/s41438-018-0083-5
文献子类	Article
英文摘要	Vitis amurensis is a wild Vitis plant that can withstand extreme cold temperatures. However, the accumulation of metabolites during cold acclimation (CA) in V. amurensis remains largely unknown. In this study, plantlets of V amurensis and V vinifera cv. Muscat of Hamburg were treated at 4 degrees C for 24 and 72 h, and changes of metabolites in leaves were detected by gas chromatography coupled with time-of-flight mass spectrometry. Most of the identified metabolites, including carbohydrates, amino acids, and organic acids, accumulated in the two types of grape after CA. Galactinol, raffinose, fructose, mannose, glycine, and ascorbate were continuously induced by cold in V amurensis, but not in Muscat of Hamburg. Twelve metabolites, including isoleucine, valine, proline, 2-oxoglutarate, and putrescine, increased in V amurensis during CA. More galactinol, ascorbate, 2-oxoglutarate, and putrescine, accumulated in V amurensis, but not in Muscat of Hamburg, during CA, which may be responsible for the excellent cold tolerance in V amurensis. The expression levels of the genes encoding (3-amylase (BAMY), galactinol synthase (GoIS), and raffinose synthase (RafS) were evaluated by quantitative reverse transcription-PCR. The expression BAMY (VIT_02s0012 g00170) and RafS (VIT_05s0077 g00840) were primarily responsible for the accumulation of maltose and raffinose, respectively. The accumulation of galactinol was attributed to different members of GoIS in the two grapes. In conclusion, these results show the inherent differences in metabolites between V amurensis and V. vinifera under CA.
学科主题	Plant Sciences ; Genetics & Heredity ; Horticulture
出版地	LONDON
WOS关键词	FREEZING TOLERANCE ; ARABIDOPSIS-THALIANA ; TRANSCRIPTION FACTOR ; INTEGRATED ANALYSIS ; GAS-CHROMATOGRAPHY ; ABIOTIC STRESS ; GENES ; SALT ; ACCESSIONS ; RESPONSES
WOS研究方向	Plant Sciences ; Genetics & Heredity ; Agriculture
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000454602600008
资助机构	Youth Innovation Promotion Association of CAS [2015281] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471857, 31672132] ; Science and Technology Service Network Initiative of CAS [KFJ-STS-ZDTP-025] ; Grape Breeding Project of Ningxia [NXNYYZ201502]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19571]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing, Peoples R China 4.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan, Hubei, Peoples R China 5.Guangzhou Univ Chinese Med, Int Inst Translat Chinese Med, Guangzhou, Guangdong, Peoples R China 6.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Chai, Fengmei,Liu, Wenwen,Xiang, Yue,et al. Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation[J]. HORTICULTURE RESEARCH,2019,6.
APA	Chai, Fengmei.,Liu, Wenwen.,Xiang, Yue.,Meng, Xianbin.,Sun, Xiaoming.,...&Li, Shaohua.(2019).Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation.HORTICULTURE RESEARCH,6.
MLA	Chai, Fengmei,et al."Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation".HORTICULTURE RESEARCH 6(2019).