A role for the Auxin Response Factors ARF6 and ARF8 homologs in petal spur elongation and nectary maturation in Aquilegia

doi:10.1111/nph.16633

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	A role for the Auxin Response Factors ARF6 and ARF8 homologs in petal spur elongation and nectary maturation in Aquilegia
	Zhang, Rui ; Min, Ya ; Holappa, Lynn D.; Walcher-Chevillet, Cristina L.; Duan, Xiaoshan 1; Donaldson, Emily ; Kong, Hongzhi ; Kramer, Elena M.
刊名	NEW PHYTOLOGIST
	2020
卷号	227 期号:5 页码:1392-1405
关键词	Aquilegia Auxin Response Factor cell elongation nectary development petal spur
ISSN号	0028-646X
DOI	10.1111/nph.16633
文献子类	Article
英文摘要	The petal spur of the basal eudicotAquilegiais a key innovation associated with the adaptive radiation of the genus. Previous studies have shown that diversification ofAquilegiaspur length can be predominantly attributed to variation in cell elongation. However, the genetic pathways that control the development of petal spurs are still being investigated. Here, we focus on a pair of closely related homologs of the AUXIN RESPONSE FACTOR family,AqARF6andAqARF8, to explore their roles inAquileiga coeruleapetal spur development. Expression analyses of the two genes show that they are broadly expressed in vegetative and floral organs, but have relatively higher expression in petal spurs, particularly at later stages. Knockdown of the twoAqARF6andAqARF8transcripts using virus-induced gene silencing resulted in largely petal-specific defects, including a significant reduction in spur length due to a decrease in cell elongation. These spurs also exhibited an absence of nectar production, which was correlated with downregulation ofSTYLISHhomologs that have previously been shown to control nectary development. This study provides the first evidence ofARF6/8homolog-mediated petal development outside the core eudicots. The genes appear to be specifically required for cell elongation and nectary maturation in theAquilegiapetal spur.
学科主题	Plant Sciences
电子版国际标准刊号	1469-8137
出版地	HOBOKEN
WOS关键词	BHLH TRANSCRIPTION FACTOR ; GENE-EXPRESSION ; KNOX GENES ; EVOLUTION ; DIVERSIFICATION ; REPRESSION ; SECRETION ; FEMALE ; FAMILY ; PLANTS
WOS研究方向	Plant Sciences
语种	英语
出版者	WILEY
WOS记录号	WOS:000539870600001
资助机构	NSFNational Science Foundation (NSF) [IOS-1456217] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930008]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21721]
专题	系统与进化植物学国家重点实验室
作者单位	1.Walcher-Chevillet, Cristina L.] 10x Genom, 6230 Stoneridge Mall Rd, Pleasanton, CA 94588 USA 2.Min, Ya; Holappa, Lynn D.; Walcher-Chevillet, Cristina L.; Donaldson, Emily; Kramer, Elena M.] Harvard Univ, Dept Organism & Evolutionary Biol, 16 Divin Ave, Cambridge, MA 02138 USA 3.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 4.Harvard Univ, Harvard Univ Herbaria, 22 Divin Ave, Cambridge, MA 02138 USA
推荐引用方式 GB/T 7714	Zhang, Rui,Min, Ya,Holappa, Lynn D.,et al. A role for the Auxin Response Factors ARF6 and ARF8 homologs in petal spur elongation and nectary maturation in Aquilegia[J]. NEW PHYTOLOGIST,2020,227(5):1392-1405.
APA	Zhang, Rui.,Min, Ya.,Holappa, Lynn D..,Walcher-Chevillet, Cristina L..,Duan, Xiaoshan.,...&Kramer, Elena M..(2020).A role for the Auxin Response Factors ARF6 and ARF8 homologs in petal spur elongation and nectary maturation in Aquilegia.NEW PHYTOLOGIST,227(5),1392-1405.
MLA	Zhang, Rui,et al."A role for the Auxin Response Factors ARF6 and ARF8 homologs in petal spur elongation and nectary maturation in Aquilegia".NEW PHYTOLOGIST 227.5(2020):1392-1405.