Exposure to hypoxia causes stress erythropoiesis and downregulates immune response genes in spleen of mice

	Exposure to hypoxia causes stress erythropoiesis and downregulates immune response genes in spleen of mice
	Wang, Haijing; Liu, Daoxin; Song, Pengfei; Jiang, Feng; Chi, Xiangwen; Zhang, Tongzuo
刊名	BMC GENOMICS
	2021
卷号	22 期号:1
关键词	Transcriptome Spleen Stress erythropoiesis Immune response Hypoxia
英文摘要	BackgroundThe spleen is the largest secondary lymphoid organ and the main site where stress erythropoiesis occurs. It is known that hypoxia triggers the expansion of erythroid progenitors; however, its effects on splenic gene expression are still unclear. Here, we examined splenic global gene expression patterns by time-series RNA-seq after exposing mice to hypoxia for 0, 1, 3, 5, 7 and 13days.ResultsMorphological analysis showed that on the 3rd day there was a significant increase in the spleen index and in the proliferation of erythroid progenitors. RNA-sequencing analysis revealed that the overall expression of genes decreased with increased hypoxic exposure. Compared with the control group, 1380, 3430, 4396, 3026, and 1636 genes were differentially expressed on days 1, 3, 5, 7 and 13, respectively. Clustering analysis of the intersection of differentially expressed genes pointed to 739 genes, 628 of which were upregulated, and GO analysis revealed a significant enrichment for cell proliferation. Enriched GO terms of downregulated genes were associated with immune cell activation. Expression of Gata1, Tal1 and Klf1 was significantly altered during stress erythropoiesis. Furthermore, expression of genes involved in the immune response was inhibited, and NK cells decreased.ConclusionsThe spleen of mice conquer hypoxia exposure in two ways. Stress erythropoiesis regulated by three transcription factors and genes in immune response were downregulated. These findings expand our knowledge of splenic transcriptional changes during hypoxia.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60792]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Haijing,Liu, Daoxin,Song, Pengfei,et al. Exposure to hypoxia causes stress erythropoiesis and downregulates immune response genes in spleen of mice[J]. BMC GENOMICS,2021,22(1).
APA	Wang, Haijing,Liu, Daoxin,Song, Pengfei,Jiang, Feng,Chi, Xiangwen,&Zhang, Tongzuo.(2021).Exposure to hypoxia causes stress erythropoiesis and downregulates immune response genes in spleen of mice.BMC GENOMICS,22(1).
MLA	Wang, Haijing,et al."Exposure to hypoxia causes stress erythropoiesis and downregulates immune response genes in spleen of mice".BMC GENOMICS 22.1(2021).