Covalently coating dextran on macroporous polyglycidyl methacrylate microsphere enabled rapid protein chromatographic separation

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	Covalently coating dextran on macroporous polyglycidyl methacrylate microsphere enabled rapid protein chromatographic separation
	Zhang, Rongyue; Li, Qiang; Li, Juan; Zhou, Weiqing; Ye, Peili; Gao, Yang; Ma, Guanghui; Su, Zhiguo
刊名	MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
	2012-12-01
卷号	32 期号:8 页码:2628-2633
关键词	Materials Hydrophilization Macroporous microsphere Rapid protein separation Covalently coating
ISSN号	0928-4931
通讯作者	Ma, GH
英文摘要	Protein denaturation and nonspecific adsorption on polymer media as a chromatographic support have been a problem which needs to be overcome. Macroporous poly(glycidyl methacrylate-divinylbezene) (PGMA-DVB) microspheres prepared in this study were firstly covalently coated with dextran through a three-step method. The dextran was firstly adsorbed onto the microspheres and then covalently bound to the PGMA-DVB microsphere through ether bonds which were formed by hydroxyl group reacting with epoxy group at the presence of 4-(Dimethylamino) pyridine. Finally, the coating dextran layer was crosslinked by ethylene glycol diglycidyl ether to form the continuous network coating. The coated microspheres were characterized by Fourier transform infrared spectra, scanning electron microscope, mercury porosimetry measurements. laser scanning confocal microscope, and protein adsorption experiments. Results showed that PGMA-DVB microspheres coated with dextran successfully maintained the macroporous structure and high permeability. The backpressure was only 1.69 MPa at a high flow rate of 2891 cm/h. Consequently, the hydrophilicity and biocompatibility of modified microspheres were greatly improved, and the contact angle decreased from 184 degrees to 13 degrees, and nonspecific adsorption of proteins was decreased to little or none. The clad dextran coating with large amounts of hydroxyl group was easily derived to be various functional groups. The derived media have great potential applications in rapid protein chromatography. (C) 2012 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Materials Science, Biomaterials
研究领域[WOS]	Materials Science
关键词[WOS]	GIGAPOROUS POLYSTYRENE MICROSPHERES ; PERFUSION CHROMATOGRAPHY ; GLYCIDYL METHACRYLATE ; CHEMICAL-MODIFICATION ; STATIONARY PHASES ; ADSORPTION ; POLYMERIZATION ; SURFACES ; ETHYL(HYDROXYETHYL)CELLULOSE ; PERFORMANCE
收录类别	SCI
语种	英语
WOS记录号	WOS:000310396900071
公开日期	2013-10-11
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3179]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Rongyue,Li, Qiang,Li, Juan,et al. Covalently coating dextran on macroporous polyglycidyl methacrylate microsphere enabled rapid protein chromatographic separation[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2012,32(8):2628-2633.
APA	Zhang, Rongyue.,Li, Qiang.,Li, Juan.,Zhou, Weiqing.,Ye, Peili.,...&Su, Zhiguo.(2012).Covalently coating dextran on macroporous polyglycidyl methacrylate microsphere enabled rapid protein chromatographic separation.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,32(8),2628-2633.
MLA	Zhang, Rongyue,et al."Covalently coating dextran on macroporous polyglycidyl methacrylate microsphere enabled rapid protein chromatographic separation".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 32.8(2012):2628-2633.