Scale-Up Preparation of Crocins I and II from Gardenia jasminoides by a Two-Step Chromatographic Approach and Their Inhibitory Activity Against ATP Citrate Lyase | |
Guan, Shuguang1,2; Pu, Qiaoli1; Liu, Yinan3; Wu, Honghong4; Yu, Wenbo1; Pi, Zifeng2,5; Liu, Shu2; Song, Fengrui2; Li, Jingya3; Guo, De-An1,3 | |
刊名 | MOLECULES |
2021-06-01 | |
卷号 | 26期号:11页码:11 |
关键词 | crocins preparation method macroporous resin column chromatography high-speed counter-current chromatography inhibitory activity on ATP citrate lyase |
DOI | 10.3390/molecules26113137 |
通讯作者 | Pi, Zifeng(mslab21@ciac.ac.cn) ; Guo, De-An(daguo@simm.ac.cn) |
英文摘要 | Crocins are highly valuable natural compounds for treating human disorders, and they are also high-end spices and colorants in the food industry. Due to the limitation of obtaining this type of highly polar compound, the commercial prices of crocins I and II are expensive. In this study, macroporous resin column chromatography combined with high-speed counter-current chromatography (HSCCC) was used to purify crocins I and II from natural sources. With only two chromatographic steps, both compounds were simultaneously isolated from the dry fruit of Gardenia jasminoides, which is a cheap herbal medicine distributed in a number of countries. In an effort to shorten the isolation time and reduce solvent usage, forward and reverse rotations were successively utilized in the HSCCC isolation procedure. Crocins I and II were simultaneously obtained from a herbal resource with high recoveries of 0.5% and 0.1%, respectively, and high purities of 98.7% and 99.1%, respectively, by HPLC analysis. The optimized preparation method was proven to be highly efficient, convenient, and cost-effective. Crocins I and II exhibited inhibitory activity against ATP citrate lyase, and their IC50 values were determined to be 36.3 +/- 6.24 and 29.7 +/- 7.41 mu M, respectively. |
资助项目 | National Standardization of Traditional Chinese Medicine Project[ZYBZH-K-LN-01] ; National Natural Science Foundation of China[81530095] ; National Natural Science Foundation of China[81673591] |
WOS关键词 | ELLIS ; PURIFICATION ; GENIPOSIDE ; SEPARATION ; CHEMISTRY ; CROCETIN ; SAFFRON ; FRUIT |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
出版者 | MDPI |
WOS记录号 | WOS:000660370800001 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/297077] |
专题 | 中国科学院上海药物研究所 |
通讯作者 | Pi, Zifeng; Guo, De-An |
作者单位 | 1.Changchun Univ Chinese Med, Coll Pharm, Changchun 130117, Peoples R China 2.Chinese Acad Sci, Changchun Inst Appl Chem, Changchun 130022, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Changchun Sunnytech Co Ltd, Changchun 130061, Peoples R China |
推荐引用方式 GB/T 7714 | Guan, Shuguang,Pu, Qiaoli,Liu, Yinan,et al. Scale-Up Preparation of Crocins I and II from Gardenia jasminoides by a Two-Step Chromatographic Approach and Their Inhibitory Activity Against ATP Citrate Lyase[J]. MOLECULES,2021,26(11):11. |
APA | Guan, Shuguang.,Pu, Qiaoli.,Liu, Yinan.,Wu, Honghong.,Yu, Wenbo.,...&Guo, De-An.(2021).Scale-Up Preparation of Crocins I and II from Gardenia jasminoides by a Two-Step Chromatographic Approach and Their Inhibitory Activity Against ATP Citrate Lyase.MOLECULES,26(11),11. |
MLA | Guan, Shuguang,et al."Scale-Up Preparation of Crocins I and II from Gardenia jasminoides by a Two-Step Chromatographic Approach and Their Inhibitory Activity Against ATP Citrate Lyase".MOLECULES 26.11(2021):11. |
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