Genetic Identification of Antimonate Respiratory Reductase in Shewanella sp. ANA-3

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Genetic Identification of Antimonate Respiratory Reductase in Shewanella sp. ANA-3
	Wang, Liying; Ye, Li; Jing, Chuanyong
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2020-11-03
卷号	54 期号:21 页码:14107-14113
ISSN号	0013-936X
英文摘要	Microbial antimonate [Sb(V)] respiratory reduction is an important process regulating Sb redox transformation in the environment. However, little is known about the microbial respiratory reductase for Sb(V). Herein, we report Sb(V)-respiring reduction by Shewanella sp. ANA-3 through an arsenate respiratory reductase encoded by arrAB. Incubation experiments showed that Shewanella sp. ANA-3 mediated Sb(V)-respiring reduction, which was dependent on the cell concentration. Both protein analysis and reverse transcriptase-polymerase chain reaction results revealed that arrAB was highly expressed in Sb(V)-respiring reduction. In vivo evidence with mutants indicated that neither ANA-3-Delta arrA nor ANA-3-Delta arrB was capable of reducing Sb(V) as efficiently as the wild type, whereas complementation by the wild-type sequences of arrA and arrB rescued the mutants' ability. Our in vitro results showed that ArrAB purified by His-Tag was able to mediate Sb(V) reduction, though with much suppressed catalytic kinetics compared with As(V) reduction. The cell-concentration-dependent reduction of Sb(V) was regulated by quorum sensing via the luxS gene. This study opens a new chapter in the mechanistic understanding of microbial Sb(V) respiratory reduction.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44699]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Wang, Liying,Ye, Li,Jing, Chuanyong. Genetic Identification of Antimonate Respiratory Reductase in Shewanella sp. ANA-3[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2020,54(21):14107-14113.
APA	Wang, Liying,Ye, Li,&Jing, Chuanyong.(2020).Genetic Identification of Antimonate Respiratory Reductase in Shewanella sp. ANA-3.ENVIRONMENTAL SCIENCE & TECHNOLOGY,54(21),14107-14113.
MLA	Wang, Liying,et al."Genetic Identification of Antimonate Respiratory Reductase in Shewanella sp. ANA-3".ENVIRONMENTAL SCIENCE & TECHNOLOGY 54.21(2020):14107-14113.