Formic Acid of ppm Enhances LC-MS/MS Detection of UV Irradiation-Induced DNA Dimeric Photoproducts

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Formic Acid of ppm Enhances LC-MS/MS Detection of UV Irradiation-Induced DNA Dimeric Photoproducts
	Zhang, Ning; Deng, Wenchao; Li, Yao; Ma, Yangde; Liu, Yan; Li, Xiangjun; Wang, Hailin
刊名	ANALYTICAL CHEMISTRY
	2020-01-07
卷号	92 期号:1 页码:1197-1204
ISSN号	0003-2700
英文摘要	Cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6-4) pyrimidone photoproducts (6-4PPs) are genotoxic DNA lesions and mainly generated on thymine-thymine (T-T) dinucleotides upon UV irradiation. Regarding the sensitivity, specificity, and accuracy of analytical methods, it is of first choice to develop a reliable assay for simultaneous detection of these DNA, lesions using liquid chromatography-tandem mass spectrometry (LC-MS/MS). However, the dilemma is the low detection sensitivity of the phosphate-containing dimeric photoproducts even using most favorable negative-ion mode for LC-MS/MS analysis. Unexpectedly, we observed that the detection sensitivity of T-T CPD and 6-4PP could be significantly improved using formic acid/acetic acid (similar to ppm) as an additive of the mobile phase for reversed-phase LC-MS/MS analysis. This is the first demonstration of the enhancement of LC-MS/MS signals by formic acid/acetic acid in negative-ion mode. Of note, these acidic agents are often used for positive-ion mode in LC-MS assays. Benefited from the developed method, we could quantify both T-T CPD and 6-4PP in mouse embryonic stem cells upon UVC irradiation at low dosage. This sensitive method is applicable to the screening and identification of genes involved in formation, signaling, and repair of UV lesion.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44695]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Zhang, Ning,Deng, Wenchao,Li, Yao,et al. Formic Acid of ppm Enhances LC-MS/MS Detection of UV Irradiation-Induced DNA Dimeric Photoproducts[J]. ANALYTICAL CHEMISTRY,2020,92(1):1197-1204.
APA	Zhang, Ning.,Deng, Wenchao.,Li, Yao.,Ma, Yangde.,Liu, Yan.,...&Wang, Hailin.(2020).Formic Acid of ppm Enhances LC-MS/MS Detection of UV Irradiation-Induced DNA Dimeric Photoproducts.ANALYTICAL CHEMISTRY,92(1),1197-1204.
MLA	Zhang, Ning,et al."Formic Acid of ppm Enhances LC-MS/MS Detection of UV Irradiation-Induced DNA Dimeric Photoproducts".ANALYTICAL CHEMISTRY 92.1(2020):1197-1204.