F-53B and PFOS treatments skew human embryonic stem cell in vitro cardiac differentiation towards epicardial cells by partly disrupting the WNT signaling pathway

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	F-53B and PFOS treatments skew human embryonic stem cell in vitro cardiac differentiation towards epicardial cells by partly disrupting the WNT signaling pathway
	Yang, Renjun; Liu, Shuyu; Liang, Xiaoxing; Yin, Nuoya; Ruan, Ting; Jiang, Linshu; Faiola, Francesco
刊名	ENVIRONMENTAL POLLUTION
	2020-06
卷号	261 页码:1-10
关键词	F-53B PFOS Human embryonic stem cells Cardiac differentiation Epicardial
ISSN号	0269-7491
英文摘要	F-53B and PFOS are two per- and polyfluoroalkyl substances (PFASs) widely utilized in the metal plating industry as mist suppressants. Recent epidemiological studies have linked PFASs to cardiovascular diseases and alterations in heart geometry. However, we still have limited understanding of the effects of F-53B and PFOS on the developing heart. In this study, we employed a human embryonic stem cell (hESC)-based cardiac differentiation system and whole transcriptomics analyses to evaluate the potential developmental cardiac toxicity of F-53B and PFOS. We utilized F-53B and PFOS concentrations of 0.1 - 60 mu M, covering the levels detected in human blood samples. We demonstrated that both F-53B and PFOS inhibited cardiac differentiation and promoted epicardial specification via upregulation of the WNT signaling pathway. Most importantly, the effects of F-53B were more robust than those of PFOS. This was because F-53B treatment disrupted the expression of more genes and led to lower cardiac differentiation efficiency. These findings imply that F-53B may not be a safe replacement for PFOS. (C) 2020 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44686]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Yang, Renjun,Liu, Shuyu,Liang, Xiaoxing,et al. F-53B and PFOS treatments skew human embryonic stem cell in vitro cardiac differentiation towards epicardial cells by partly disrupting the WNT signaling pathway[J]. ENVIRONMENTAL POLLUTION,2020,261:1-10.
APA	Yang, Renjun.,Liu, Shuyu.,Liang, Xiaoxing.,Yin, Nuoya.,Ruan, Ting.,...&Faiola, Francesco.(2020).F-53B and PFOS treatments skew human embryonic stem cell in vitro cardiac differentiation towards epicardial cells by partly disrupting the WNT signaling pathway.ENVIRONMENTAL POLLUTION,261,1-10.
MLA	Yang, Renjun,et al."F-53B and PFOS treatments skew human embryonic stem cell in vitro cardiac differentiation towards epicardial cells by partly disrupting the WNT signaling pathway".ENVIRONMENTAL POLLUTION 261(2020):1-10.