Cytotoxicity of hexabromocyclododecane, 1,2-dibromo-4-(1,2-dibromoethyl) cyclohexane and 1,2,5,6-tetrabromocyclooctane in human SH-SY5Y neuroblastoma cells

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Cytotoxicity of hexabromocyclododecane, 1,2-dibromo-4-(1,2-dibromoethyl) cyclohexane and 1,2,5,6-tetrabromocyclooctane in human SH-SY5Y neuroblastoma cells
	Shi, Xiaoli; Wen, Bei; Huang, Honglin; Zhang, Shuzhen
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2020-10-15
卷号	739 页码:1-16
关键词	Neurotoidcity Apoptosis Cycloaliphatic brominated flame retardant Reactive oxygen species Intracellular calcium
ISSN号	0048-9697
英文摘要	With the listing of the of cycloaliphatic brominated flame retardants (CBFR) hexabromocyclododecane (HBCD) as a persistent organic pollutant (POP) by the Stockholm Convention, much attention has been paid to the environmental behaviors and biological effects of HBCD, as well as its potential alternatives, such as 1,2-dibromo-4-(1,2-dibromoethyl) cyclohexane (TBECH) and 1,2,5,6-tetrabromocyclooctane (TBCO). In this study, the neurotoxicity of HBCD, TBECH, and TBCO in human SH-SY5Y cells were compared. The results showed that HBCD, TBECH, and TBCO induced cytotoxicity, including dose-dependent cell viability decreases, cell membrane permeability increases, cytoskeleton development damage, and apoptosis induction, with the cytotoxicity in the order of HBCD > TBCO > TBECH. The expression levels of apoptotic proteins (caspase-3, Bax, caspase-9, Bcl-2, and cytochrome c (Cyt c)) followed the same order, which indicated that mitochondrial apoptotic pathway may be one of the mechanisms responsible for their neurotoxicity. In order to study the mechanisms of cytotoxicity, CBFRsinduced reactive oxygen species (ROS) and the intracellular calcium levels were determined. The ROS levels were significantly elevated for three CBFRs treatment, suggesting that oxidative stress contributes to their cytotoxicity. The intracellular calcium concentrations were significantly enhanced for HBCD and TBCO treatment, but not for TBECH, indicating that in addition to ROS, cytotoxicity of HBCD and TBCO may follow Ca2+ -mediated apoptotic pathway. This study first compared the neurotoxicity of different CBFRs, providing valuable information for their risk assessment. (C) 2020 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44649]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Shi, Xiaoli,Wen, Bei,Huang, Honglin,et al. Cytotoxicity of hexabromocyclododecane, 1,2-dibromo-4-(1,2-dibromoethyl) cyclohexane and 1,2,5,6-tetrabromocyclooctane in human SH-SY5Y neuroblastoma cells[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,739:1-16.
APA	Shi, Xiaoli,Wen, Bei,Huang, Honglin,&Zhang, Shuzhen.(2020).Cytotoxicity of hexabromocyclododecane, 1,2-dibromo-4-(1,2-dibromoethyl) cyclohexane and 1,2,5,6-tetrabromocyclooctane in human SH-SY5Y neuroblastoma cells.SCIENCE OF THE TOTAL ENVIRONMENT,739,1-16.
MLA	Shi, Xiaoli,et al."Cytotoxicity of hexabromocyclododecane, 1,2-dibromo-4-(1,2-dibromoethyl) cyclohexane and 1,2,5,6-tetrabromocyclooctane in human SH-SY5Y neuroblastoma cells".SCIENCE OF THE TOTAL ENVIRONMENT 739(2020):1-16.