Application of electrophysiological technique in toxicological study: From manual to automated patch-clamp recording

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Application of electrophysiological technique in toxicological study: From manual to automated patch-clamp recording
	Gao, Jia; Zhang, He; Xiong, Ping; Yan, Xueting; Liao, Chunyang; Jiang, Guibin
刊名	TRAC-TRENDS IN ANALYTICAL CHEMISTRY
	2020-12
卷号	133 页码:1-9
关键词	Manual patch-clamp Automated patch-clamp Ion channel characterization High throughput screening Toxicity
ISSN号	0165-9936
英文摘要	Ion channels are common targets of a range of environmental substances, including pharmaceuticals, toxins, metals and many organic pollutants. Patch-clamp technique is gold standard in studying ion channel characteristics, contributing to the improved accuracy in active compound identification, the increasing studies of molecular mechanism behind diseases, and the better prediction of potential toxicants. Nevertheless, low throughput and high technical capability requirement limit the application of conventional manual patch-clamp recording and an emerging transformation towards automation and simplification is rising. The evolving automated patch-clamp technique shows obvious advances over manual technique in many aspects, including reproducibility, throughput, operability and standardization. The application of emerging automated patch-clamp platform has created valuable consequences in many research areas, especially in high throughput screening. In this study, we provided an overview on the progresses made by conventional manual and emerging automated patch-clamp techniques in toxicological studies. (C) 2020 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44607]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Gao, Jia,Zhang, He,Xiong, Ping,et al. Application of electrophysiological technique in toxicological study: From manual to automated patch-clamp recording[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2020,133:1-9.
APA	Gao, Jia,Zhang, He,Xiong, Ping,Yan, Xueting,Liao, Chunyang,&Jiang, Guibin.(2020).Application of electrophysiological technique in toxicological study: From manual to automated patch-clamp recording.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,133,1-9.
MLA	Gao, Jia,et al."Application of electrophysiological technique in toxicological study: From manual to automated patch-clamp recording".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 133(2020):1-9.