Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein

	Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein
	Zhang, Wei 1; He, Xi-Wen 1; Chen, Yang 1; Li, Wen-You 1; Zhang, Yu-Kui 1,2
刊名	biosensors & bioelectronics
	2012-01-15
卷号	31 期号:1 页码:84-89
关键词	Molecular imprinting Quantum dots Denatured bovine serum albumin Artificial receptors Selective protein recognition
通讯作者	李文友
产权排序	2,5
英文摘要	a new type of molecularly imprinted polymer (mip)-based fluorescent artificial receptor was developed by anchoring mip on the surface of denatured bovine serum albumin (dbsa) modified cdte quantum dots (qds) using the surface molecular imprinting process. the approach combined the merits of molecular imprinting technology and the fluorescent property of the cdte qds. the dbsa was used not only to modify the surface defects of the cdte qds, but also as assistant monomer to create effective recognition sites. three different proteins, namely lysozyme (lyz), cytochrome c (cyt) and methylated bovine serum albumin (mbsa), were tested as the template molecules and then the receptors were synthesized by sol-gel reaction (imprinting process). the results of fluorescence and binding experiments demonstrated the recognition performance of the receptors toward the corresponding template. under optimum conditions, the linear range for lyz was from 1.4 x 10(-8) to 8.5 x 10(-6) m, and the detection limit was 6.8 nm. moreover, the new artificial receptors were applied to separate and detect lyz in real samples. this fluorescent artificial receptor may serve as a starting point in the design of highly effective synthetic fluorescent receptor for recognition of target protein. (c) 2011 elsevier b.v. all rights reserved.
学科主题	物理化学
类目[WOS]	biophysics ; biotechnology & applied microbiology ; chemistry, analytical ; electrochemistry ; nanoscience & nanotechnology
研究领域[WOS]	biophysics ; biotechnology & applied microbiology ; chemistry ; electrochemistry ; science & technology - other topics
关键词[WOS]	nanoparticles ; nanocrystals ; sensor ; water ; photoluminescence ; biomolecules ; lysozyme ; system
收录类别	SCI
语种	英语
WOS记录号	WOS:000300468400013
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118297]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wei,He, Xi-Wen,Chen, Yang,et al. Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein[J]. biosensors & bioelectronics,2012,31(1):84-89.
APA	Zhang, Wei,He, Xi-Wen,Chen, Yang,Li, Wen-You,&Zhang, Yu-Kui.(2012).Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein.biosensors & bioelectronics,31(1),84-89.
MLA	Zhang, Wei,et al."Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein".biosensors & bioelectronics 31.1(2012):84-89.