A poly(ethylene glycol)-brush decorated magnetic polymer for highly specific enrichment of phosphopeptides

	A poly(ethylene glycol)-brush decorated magnetic polymer for highly specific enrichment of phosphopeptides
	Zhao, Liang; Qin, Hongqiang; Hu, Zhengyan; Zhang, Yi; Wu, Ren'an; Zou, Hanfa
刊名	chemical science
	2012
卷号	3 期号:9 页码:2828-2838
ISSN号	2041-6520
通讯作者	吴仁安 ; 邹汉法
产权排序	1,1
英文摘要	immobilized metal affinity chromatography (imac) is a powerful method in phosphopeptide enrichment. however, the achievement of highly specific enrichment and sensitive detection of phosphopeptide by imac is still a big challenge because of the lack of high specificity and large binding capacity of conventional imac materials. here, we report a novel imac nanoparticle to dramatically improve the enrichment specificity for the phosphopeptide by introducing a titanium phosphate moiety on a poly(ethylene glycol) methacrylate (peg) brush decorated fe3o4@sio2 core-shell nanoparticle (denoted as fe3o4@sio2@peg-ti4+ imac nanoparticle). the thicker grafting layer of the peg brushes has a higher chelating capacity of titanium ions. due to the combination of the superior nonfouling property and the enhanced binding capacity of the grafted peg brushes, the fe3o4@sio2@peg-ti4+ imac nanoparticle demonstrated a high phosphopeptide recovery (over 70%) and low limit of detection (0.5 fmol), along with an exceptional great specificity to capture phosphopeptides from a tryptic digest of the mixture of a nonphosphorylated protein bsa and a phosphorylated protein alpha-casein with molar ratios of bsa/alpha-casein up to 2000 : 1. in the analysis of a real complex biological sample, the tryptic digests of arabidopsis, 2447 unique phosphopeptides have been identified, showing a superior performance of the fe3o4@sio2@peg-ti4+ imac nanoparticle than that of fe3o4@sio2-ti4+ (1186) and commercial tio2 microspheres (961). we believe that the peg decoration for imac materials will be a convenient approach to significantly improve the specificity and the binding capacity of phosphopeptide enrichment.
学科主题	物理化学
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	metal affinity-chromatography ; mass-spectrometry analysis ; maldi-tof-ms ; multi-phosphorylated peptides ; phosphoproteome analysis ; biomedical applications ; radical polymerization ; selective enrichment ; silica microspheres ; rapid enrichment
收录类别	SCI
语种	英语
WOS记录号	WOS:000306931300023
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118291]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Liang,Qin, Hongqiang,Hu, Zhengyan,et al. A poly(ethylene glycol)-brush decorated magnetic polymer for highly specific enrichment of phosphopeptides[J]. chemical science,2012,3(9):2828-2838.
APA	Zhao, Liang,Qin, Hongqiang,Hu, Zhengyan,Zhang, Yi,Wu, Ren'an,&Zou, Hanfa.(2012).A poly(ethylene glycol)-brush decorated magnetic polymer for highly specific enrichment of phosphopeptides.chemical science,3(9),2828-2838.
MLA	Zhao, Liang,et al."A poly(ethylene glycol)-brush decorated magnetic polymer for highly specific enrichment of phosphopeptides".chemical science 3.9(2012):2828-2838.