Depletion of Acidic Phosphopeptides by SAX To Improve the Coverage for the Detection of Basophilic Kinase Substrates

	Depletion of Acidic Phosphopeptides by SAX To Improve the Coverage for the Detection of Basophilic Kinase Substrates
	Dong, Mingming 1,2; Ye, Mingliang 1; Cheng, Kai 1,2; Song, Chunxia 1,2; Pan, Yanbo 1,2; Wang, Chunli 1,2; Bian, Yangyang 1,2; Zou, Hanfa 1
刊名	journal of proteome research
	2012-09-01
卷号	11 期号:9 页码:4673-4681
关键词	phosphoproteome analysis SAX depletion of acidic phosphopeptides basophilic kinase substrates
ISSN号	1535-3893
通讯作者	叶明亮 ; 邹汉法
产权排序	1,1
英文摘要	the ser/thr protein kinases fall into three major subgroups, pro-directed, basophilic, and acidophilic, on the basis of the types of substrate sequences that they preferred. despite many phosphoproteomics efforts that have been taken for global profiling of phosphopeptides, methodologies focusing on analyzing a particular type of kinase substrates have seldom been reported. selective enrichment of phosphopeptides from basophilic kinase substrates is difficult because basophilic motifs are cleaved by trypsin during digestion. in this study, we develop a negative enrichment strategy to enhance the identification of basophilic kinase substrates. this method is based on an observation that high ph strong anion exchange (sax) chromatography can separate tryptic phosphopeptides according to the number of acidic amino acidic residues that they have. thus, sax was applied to deplete acidic phosphopeptides from the phosphopeptide mixture, which improved the coverage for the detection of basophilic kinase substrates. the sax depletion approach was further combined with online scx-rp separation for large-scale analysis of mouse liver phosphoproteome, which resulted in the identification of 6944 phosphorylated sites. it was found that motifs associated with basophilic kinases prevail for these identified phosphorylated sites.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	ion affinity-chromatography ; titanium-dioxide microcolumns ; mass-spectrometric analysis ; protein-phosphorylation ; phosphoproteome analysis ; signaling networks ; in-vivo ; enrichment ; peptides ; identification
收录类别	SCI
语种	英语
WOS记录号	WOS:000308389900025
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118241]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Dong, Mingming,Ye, Mingliang,Cheng, Kai,et al. Depletion of Acidic Phosphopeptides by SAX To Improve the Coverage for the Detection of Basophilic Kinase Substrates[J]. journal of proteome research,2012,11(9):4673-4681.
APA	Dong, Mingming.,Ye, Mingliang.,Cheng, Kai.,Song, Chunxia.,Pan, Yanbo.,...&Zou, Hanfa.(2012).Depletion of Acidic Phosphopeptides by SAX To Improve the Coverage for the Detection of Basophilic Kinase Substrates.journal of proteome research,11(9),4673-4681.
MLA	Dong, Mingming,et al."Depletion of Acidic Phosphopeptides by SAX To Improve the Coverage for the Detection of Basophilic Kinase Substrates".journal of proteome research 11.9(2012):4673-4681.