A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems

	A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems
	Geng, Xuhui 1,2; Wu, Dapeng 1; Guan, Yafeng 1
刊名	talanta
	2012-01-15
卷号	88 期号:15 页码:463-467
关键词	Light-emitting diode Fluorescence detector Compact
通讯作者	关亚风
产权排序	1,1
英文摘要	a compact and highly sensitive light-emitting diode-induced fluorescence detector (led-fd) was constructed and evaluated. the led excitation beam was restricted by a 200 mu m diameter pinhole on a 55 mu m thick lighttight film. the led, excitation filter, pinhole, and capillary flow cell were placed tightly against each other without either optical lens or excitation fiber. the performance of the led-fd was evaluated by flow injection analysis (fia) of sodium fluorescein solution. the limit of detection (lod) was determined as 0.75 nm (snr= 3). compared with our previous work, a 3.5-fold enhancement on the signal-to-noise ratio (snr) was obtained. the system exhibited linear responses in the concentration range of 2-200 nm (r = 0.9993). the error of repeatability was within 2.0% r.s.d. on peak height in a ten-repeated injection of 5 nm solution. the reproducibility between devices was within 2.2% r.s.d. on peak height of 10 nm fluorescein solution. finally, the led-fd was compared with commercial fluorescence detector, and similar fluorescence signal intensity was obtained. (c) 2011 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	optical-fiber ; electrophoresis ; performance
收录类别	SCI
语种	英语
WOS记录号	WOS:000301159400064
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118224]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Geng, Xuhui,Wu, Dapeng,Guan, Yafeng. A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems[J]. talanta,2012,88(15):463-467.
APA	Geng, Xuhui,Wu, Dapeng,&Guan, Yafeng.(2012).A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems.talanta,88(15),463-467.
MLA	Geng, Xuhui,et al."A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems".talanta 88.15(2012):463-467.