Taxol-induced alteration of intracellular amino-acid profile related to human cervical carcinoma HeLa cell death

	Taxol-induced alteration of intracellular amino-acid profile related to human cervical carcinoma HeLa cell death
	Han, Xiao-Fei; Liu, Yan-Qiu; Wang, Long-Xing; Yang, Qian-Xu; Xiao, Hong-Bin
刊名	biotechnology letters
	2012
卷号	34 期号:1 页码:1-7
关键词	L-Alanine Intracellular amino-acid profile Human cervical carcinoma HeLa cell Taxol
通讯作者	肖红斌
产权排序	1,1
英文摘要	the anti-tumor action of taxol was investigated in the changes of amino-acids involved in tumor cell survival. by tracing the intracellular amino-acid profiles of hela cells treated with non-conditioned and three conditioned media (taxol, l-alanine, and taxol + l-alanine), it was observed that an alteration of amino-acid metabolism participates in taxol-induced death of hela cells. the contents of 18 out of 21 detected amino-acids are 5-95% and the ones of lysine and methionine are 158 and 117% of the corresponding contents in the control after treatment with taxol for 24 h, respectively. addition of l-alanine inhibited cell apoptosis upon taxol treatment by partially blocking the increase of lysine and methionine and reversing decrease trend of alanine, glycine, and glutamic acid. these results suggest that interference of amino-acid metabolism might be an important mechanism of taxol cytotoxicity.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biotechnology & applied microbiology
研究领域[WOS]	biotechnology & applied microbiology
关键词[WOS]	induced apoptosis ; cancer cells ; growth ; lysine ; deprivation ; imbalance
收录类别	SCI
语种	英语
WOS记录号	WOS:000297862100001
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118191]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Han, Xiao-Fei,Liu, Yan-Qiu,Wang, Long-Xing,et al. Taxol-induced alteration of intracellular amino-acid profile related to human cervical carcinoma HeLa cell death[J]. biotechnology letters,2012,34(1):1-7.
APA	Han, Xiao-Fei,Liu, Yan-Qiu,Wang, Long-Xing,Yang, Qian-Xu,&Xiao, Hong-Bin.(2012).Taxol-induced alteration of intracellular amino-acid profile related to human cervical carcinoma HeLa cell death.biotechnology letters,34(1),1-7.
MLA	Han, Xiao-Fei,et al."Taxol-induced alteration of intracellular amino-acid profile related to human cervical carcinoma HeLa cell death".biotechnology letters 34.1(2012):1-7.