Isolation and structural characterization of the polysaccharide LRGP1 from Lycium ruthenicum

	Isolation and structural characterization of the polysaccharide LRGP1 from Lycium ruthenicum
	Peng, Qiang 2,3; Lv, Xiaopeng 1; Xu, Qingsong 2; Li, Yu 2,3; Huang, Linjuan 1; Du, Yuguang 2
刊名	carbohydrate polymers
	2012-09-01
卷号	90 期号:1 页码:95-101
关键词	Lycium ruthenicum Polysaccharide Structure Glycoconjugate Arabinogalactan-proteins
通讯作者	linjuanhuang ; 杜昱光
产权排序	1,1
英文摘要	a novel water-soluble glycoconjugate, designated as lycium ruthenicum glycoconjugate polysaccharide 1 (lrgp1), was isolated from the fruits of lycium ruthenicum murr. the crude polysaccharide was obtained by hot water extraction and purified by ion-exchange and gelfiltration chromatography. its molecular weight was 56.2 kda determined by hpgpc (high performance gel permeation chromatography). monosaccharide composition analysis revealed that it was composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 0.65:10.71:0.33:0.67:1:10.41. the existence of o-type carbohydrate-peptide linkage in lrgp1 was demonstrated by beta-elimination reaction. on the basis of monosaccharide composition, partial acid hydrolysis, methylation analysis and esi-ms analysis, lrgp1 was characterized as a branched polysaccharide rich in arabinose and galactose with a backbone composed of (1 -> 3)-linked gal. the branches were composed of (1 -> 5)-linked ara, (1 -> 2)-linked ara, (1 -> 6)-linked gal, (1 -> 3)-linked gal, (1 -> 4)-linked gal and (1 -> 2,4)-linked rha. arabinose, xylose, mannose, and glucose were located at the terminal of the branches. (c) 2012 elsevier ltd. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, applied ; chemistry, organic ; polymer science
研究领域[WOS]	chemistry ; polymer science
关键词[WOS]	cell-wall ; purification ; arabinogalactans ; glycoprotein ; polymers ; barbarum ; protein
收录类别	SCI
语种	英语
WOS记录号	WOS:000308267000013
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118084]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.NW Univ Xian, Life Sci Coll, Educ Minist, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Peng, Qiang,Lv, Xiaopeng,Xu, Qingsong,et al. Isolation and structural characterization of the polysaccharide LRGP1 from Lycium ruthenicum[J]. carbohydrate polymers,2012,90(1):95-101.
APA	Peng, Qiang,Lv, Xiaopeng,Xu, Qingsong,Li, Yu,Huang, Linjuan,&Du, Yuguang.(2012).Isolation and structural characterization of the polysaccharide LRGP1 from Lycium ruthenicum.carbohydrate polymers,90(1),95-101.
MLA	Peng, Qiang,et al."Isolation and structural characterization of the polysaccharide LRGP1 from Lycium ruthenicum".carbohydrate polymers 90.1(2012):95-101.