Depth-recognizable time-domain fluorescence molecular tomography in reflective geometry

doi:10.1364/BOE.430235

CORC > 自动化研究所 > 中国科学院自动化研究所 > 中国科学院分子影像重点实验室

	Depth-recognizable time-domain fluorescence molecular tomography in reflective geometry
	Cheng, Jiaju 1; Zhang, Peng 2,3; Cai, Chuangjian 1; Gao, Yang 1; Liu, Jie 2; Hui, Hui 3; Tian, Jie 3,4; Luo, Jianwen 1
刊名	BIOMEDICAL OPTICS EXPRESS
	2021-07-01
卷号	12 期号:7 页码:3806-3818
ISSN号	2156-7085
DOI	10.1364/BOE.430235
通讯作者	Luo, Jianwen(luo_jianwen@tsinghua.edu.cn)
英文摘要	Fluorescence molecular tomography (FMT) is a functional imaging modality which is capable of noninvasively detecting in-vivo biological activities on molecular level. It provides quantitatively accurate three-dimensional (3D) distributions of endogenous or exogenous fluorescent biomarkers in thick and highly scattering media such as biological tissues. Therefore, FMT is a vital tool in preclinical oncology research, drug development and other biological research areas [1-5]. Despite its advantages of non-ionizing radiation and low cost, the emerging clinical applications of FMT have been hampered not only by its low spatial resolution caused by the ill-posed nature Conventional fluorescence molecular tomography (FMT) reconstruction requires photons penetrating the whole object, which limits its applications to small animals. However, by utilizing reflective photons, fluorescence distribution near the surface could be reconstructed regardless of the object size, which may extend the applications of FMT to surgical navigation and so on. Therefore, time-domain reflective fluorescence molecular tomography (TD-rFMT) is proposed in this paper. The system excites and detects the emission light from the same angle within a field of view of 5 cm. Because the detected intensities of targets depend strongly on the depth, the reconstruction of targets in deep regions would be evidently affected. Therefore, a fluorescence yield reconstruction method with depth regularization and a weighted separation reconstruction strategy for lifetime are developed to enhance the performance for deep targets. Through simulations and phantom experiments, TD-rFMT is proved capable of reconstructing fluorescence distribution within a 2.5-cm depth with accurate reconstructed yield, lifetime, and target position(s).
资助项目	National Natural Science Foundation of China (NSFC)[61871022] ; National Natural Science Foundation of China (NSFC)[61871251] ; National Natural Science Foundation of China (NSFC)[61871263] ; National Natural Science Foundation of China (NSFC)[62027901]
WOS关键词	L1 REGULARIZATION ; RECONSTRUCTION METHOD ; MOUSE MODEL
WOS研究方向	Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	OPTICAL SOC AMER
WOS记录号	WOS:000671937600005
资助机构	National Natural Science Foundation of China (NSFC)
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/45267]
专题	自动化研究所_中国科学院分子影像重点实验室
通讯作者	Luo, Jianwen
作者单位	1.Tsinghua Univ, Sch Med, Dept Biomed Engn, Beijing 100084, Peoples R China 2.Beijing Jiaotong Univ, Sch Comp & Informat Technol, Dept Biomed Engn, Beijing 100044, Peoples R China 3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China 4.Beihang Univ, Beijing Adv Innovat Ctr Big Data Based Precis Med, Sch Med, Beijing 100191, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Jiaju,Zhang, Peng,Cai, Chuangjian,et al. Depth-recognizable time-domain fluorescence molecular tomography in reflective geometry[J]. BIOMEDICAL OPTICS EXPRESS,2021,12(7):3806-3818.
APA	Cheng, Jiaju.,Zhang, Peng.,Cai, Chuangjian.,Gao, Yang.,Liu, Jie.,...&Luo, Jianwen.(2021).Depth-recognizable time-domain fluorescence molecular tomography in reflective geometry.BIOMEDICAL OPTICS EXPRESS,12(7),3806-3818.
MLA	Cheng, Jiaju,et al."Depth-recognizable time-domain fluorescence molecular tomography in reflective geometry".BIOMEDICAL OPTICS EXPRESS 12.7(2021):3806-3818.