Short-chain peptides identification of scorpion Buthus martensi Karsch venom by employing high orthogonal 2D-HPLC system and tandem mass spectrometry

	Short-chain peptides identification of scorpion Buthus martensi Karsch venom by employing high orthogonal 2D-HPLC system and tandem mass spectrometry
	Xu, Junyan 1,2; Zhang, Xiuli 1; Guo, Zhimou 1; Yan, Jingyu 1; Yu, Long 1; Li, Xiuling 1; Xue, Xingya 1; Liang, Xinmiao 1
刊名	proteomics
	2012-10-01
卷号	12 期号:19-20 页码:3076-3084
关键词	Hydrophilic interaction chromatography Peptide separation Scorpion venom Tandem mass spectrometry Two-dimensional liquid chromatography
通讯作者	梁鑫淼
产权排序	1,1
英文摘要	scorpion venom contains a considerable variety of neurotoxic peptides that can act on ionic channels. here, we describe an orthogonal 2d-reversed phase/hydrophilic interaction chromatography system (rplc/hilic) and use it to separate short-chain peptides from asian scorpion buthus martensi karsch (bmk) venom in a high throughput format. due to its high orthogonality and efficiency, 18 homogenous peptides were purified and sequence identified by ms/ms with collision-induced dissociation. among them, four peptides were discovered, which only have evidence at transcript-level, were first purified from crude venom in this study. two peptides named bmkk2-b and martentoxin-b were found the new cleaved chains of known bmkk2 and martentoxin. in addition, two novel peptides named bmkk12 and bmkk16 in this paper were sequenced by de novo ms/ms, which we predict, are members of potassium channel toxin a-ktx 17 subfamily by homology to other known peptides found in the swiss-prot protein database.
学科主题	物理化学
类目[WOS]	biochemical research methods ; biochemistry & molecular biology
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	k+ channel ; pharmacological characterization ; liquid-chromatography ; potassium channels ; proteomic analysis ; rat-brain ; hilic-rp ; purification ; toxins ; separation
收录类别	SCI
语种	英语
WOS记录号	WOS:000310076300017
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/117976]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Liaoning, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xu, Junyan,Zhang, Xiuli,Guo, Zhimou,et al. Short-chain peptides identification of scorpion Buthus martensi Karsch venom by employing high orthogonal 2D-HPLC system and tandem mass spectrometry[J]. proteomics,2012,12(19-20):3076-3084.
APA	Xu, Junyan.,Zhang, Xiuli.,Guo, Zhimou.,Yan, Jingyu.,Yu, Long.,...&Liang, Xinmiao.(2012).Short-chain peptides identification of scorpion Buthus martensi Karsch venom by employing high orthogonal 2D-HPLC system and tandem mass spectrometry.proteomics,12(19-20),3076-3084.
MLA	Xu, Junyan,et al."Short-chain peptides identification of scorpion Buthus martensi Karsch venom by employing high orthogonal 2D-HPLC system and tandem mass spectrometry".proteomics 12.19-20(2012):3076-3084.