A new method for quantitative analysis of cell surface glycoproteome

	A new method for quantitative analysis of cell surface glycoproteome
	Sun, Zhen; Chen, Rui; Cheng, Kai; Liu, Hongwei; Qin, Hongqiang; Ye, Mingliang; Zou, Hanfa
刊名	proteomics
	2012-11-01
卷号	12 期号:22 页码:3328-3337
关键词	Cell surface glycoprotein CD-annotated protein Glycopeptides Glycoproteomics Non-glycopeptides Quantification
ISSN号	1615-9853
通讯作者	邹汉法
产权排序	1,1
英文摘要	as the altered glycosylation expressions of cell surface proteins are associated with many diseases, glycoproteomics approach has been widely applied to characterization of surface glycosylation alteration. in general, the abundances of proteolytic glycopeptides derived from corresponding glycoproteins can be measured to determine the abundances of glycoproteins. however, this quantification strategy cannot distinguish whether the changes are results from changes of protein abundance or changes in glycosite occupancy. for the accurate and specific quantification of the cell surface glycosylation profile, we proposed a modified cell surface-capturing strategy where the glycopeptides were submitted to lc-ms/ms analysis directly for identification of glycoproteins and the non-glycopeptides were isotopically labelled for quantification of glycoproteins. this strategy was applied to comparatively analyze cell surface glycoproteins of two human cell lines, i.e. chang liver and hepg2 cells. totally 341 glycoproteins were identified with 82.4% specificity for cell membrane proteins and 33 glycoproteins were quantified with significant expression change between the two cell lines. the differential expressions of two selected proteins (emmprin and bcam) were validated by western blotting. this method enables specific and accurate analysis of the cell surface glycoproteins and may have broad application in the field of biomarker and drug target discovery.
学科主题	物理化学
类目[WOS]	biochemical research methods ; biochemistry & molecular biology
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	collagenase-stimulatory factor ; mass-spectrometry ; site-occupancy ; hydrazide chemistry ; membrane proteome ; n-glycosylation ; chromatography ; proteins ; identification ; quantification
收录类别	SCI
语种	英语
WOS记录号	WOS:000311616000007
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/117934]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Sun, Zhen,Chen, Rui,Cheng, Kai,et al. A new method for quantitative analysis of cell surface glycoproteome[J]. proteomics,2012,12(22):3328-3337.
APA	Sun, Zhen.,Chen, Rui.,Cheng, Kai.,Liu, Hongwei.,Qin, Hongqiang.,...&Zou, Hanfa.(2012).A new method for quantitative analysis of cell surface glycoproteome.proteomics,12(22),3328-3337.
MLA	Sun, Zhen,et al."A new method for quantitative analysis of cell surface glycoproteome".proteomics 12.22(2012):3328-3337.