Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework

doi:10.1038/s41467-020-14664-8

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework
	Song, P ; Shen, JW ; Ye, DK ; Dong, BJ ; Wang, F ; Pei, H ; Wang, JB ; Shi, JY ; Wang, LH ; Xue, W
刊名	NATURE COMMUNICATIONS
	2020
卷号	11 期号:1 页码:-
关键词	GLUCOSE-RESPONSIVE INSULIN PROSTATE-CANCER SARCOSINE CELLS NANOSTRUCTURES METABOLISM LIPOSOMES MECHANISM BIOMARKER MACHINES
ISSN号	2041-1723
DOI	10.1038/s41467-020-14664-8
文献子类	期刊论文
英文摘要	Protein-protein interactions are spatially regulated in living cells to realize high reaction efficiency, as seen in naturally existing electron-transfer chains. Nevertheless, arrangement of chemical/biochemical components at the artificial device interfaces does not possess the same level of control. Here we report a tetrahedral DNA framework-enabled bulk enzyme heterojunction (BEH) strategy to program the multi-enzyme catalytic cascade at the interface of electrochemical biosensors. The construction of interpenetrating network of BEH at the millimeter-scale electrode interface brings enzyme pairs within the critical coupling length (CCL) of similar to 10nm, which in turn greatly improve the overall catalytic cascade efficiency by similar to 10-fold. We demonstrate the BEH generality with a range of enzyme pairs for electrochemically detecting clinically relevant molecular targets. As a proof of concept, a BEH-based sarcosine sensor enables single-step detection of the metabolic biomarker of sarcosine with ultrasensitivity, which hold the potential for precision diagnosis of early-stage prostate cancer.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/33089]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.East China Normal Univ, Shanghai Key Lab Green Chem & Chem Proc, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China 2.Shanghai Jiao Tong Univ, Inst Mol Med, Dept Urol, Dept Nucl Med,Renji Hosp,Sch Med, Shanghai 200127, Peoples R China 3.Shanghai Jiao Tong Univ, Frontiers Sci Ctr Transformat Mol, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China 4.East China Normal Univ, Shanghai Key Lab Regulatory Biol, Inst Biomed Sci, Sch Life Sci, Shanghai 200241, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Div Phys Biol, Shanghai 201800, Peoples R China 6.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Bioimaging Ctr,Shanghai Synchrotron Radiat Facil, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Song, P,Shen, JW,Ye, DK,et al. Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework[J]. NATURE COMMUNICATIONS,2020,11(1):-.
APA	Song, P.,Shen, JW.,Ye, DK.,Dong, BJ.,Wang, F.,...&Fan, CH.(2020).Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework.NATURE COMMUNICATIONS,11(1),-.
MLA	Song, P,et al."Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework".NATURE COMMUNICATIONS 11.1(2020):-.