Microalgal motility measurement microfluidic chip for toxicity assessment of heavy metals

	Microalgal motility measurement microfluidic chip for toxicity assessment of heavy metals
	Zheng, Guoxia 1,2; Wang, Yunhua 2; Qin, Jianhua 1
刊名	analytical and bioanalytical chemistry
	2012-12-01
卷号	404 期号:10 页码:3061-3069
关键词	Microfluidic chip Marine microalgae Motility Toxicity assessment Chemostatic module
通讯作者	秦建华
产权排序	1,1
英文摘要	a polydimethylsiloxane microfluidic chip has been developed for the estimation of toxic heavy metals based on measurement of mobility of marine microalgae. the chip is mainly composed of an upstream concentration gradient generator and a downstream perfusion-based chemotatic module. the processes of toxic liquid dilution and diffusion, microalgal culturing, cell stimulation, and online screening can be integrated in this chip, which makes it an attractive approach to simplify toxicity testing procedures. the microalgal motility was adopted as a microfluidic bioassay signal and was evaluated as the percentage of motile cells, curvilinear velocity, average path velocity, and straight line velocity. two mobile marine microalgae, platymonas subcordiformis and platymonas helgolandica var. tsingtaoensis, were confined in the chemotatic module and stimulated by the eight concentration gradients of cu and cd generated by the concentration gradient generator. in all cases, a toxic response was detected (i.e., a dose-related inhibition of motility was observed). only 1.5 h was needed to predict ec50 values. thus, the microfluidic chip developed was proved to be useful as a simple and rapid approach in heavy metal detection and might be expanded as a conventional test method in environmental toxicity assessment.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	marine microalgae ; sperm motility ; algae ; copper ; cadmium ; systems ; pollutants ; biosensor ; bacterial ; growth
收录类别	SCI
语种	英语
WOS记录号	WOS:000311310100028
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/117872]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Dalian 116622, Liaoning, Peoples R China 2.Dalian Univ, Dalian 116622, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Guoxia,Wang, Yunhua,Qin, Jianhua. Microalgal motility measurement microfluidic chip for toxicity assessment of heavy metals[J]. analytical and bioanalytical chemistry,2012,404(10):3061-3069.
APA	Zheng, Guoxia,Wang, Yunhua,&Qin, Jianhua.(2012).Microalgal motility measurement microfluidic chip for toxicity assessment of heavy metals.analytical and bioanalytical chemistry,404(10),3061-3069.
MLA	Zheng, Guoxia,et al."Microalgal motility measurement microfluidic chip for toxicity assessment of heavy metals".analytical and bioanalytical chemistry 404.10(2012):3061-3069.