Ion-induced PCR strategy for mercury detection

doi:10.1002/elps.201900324

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 国家天然药物工程技术研究中心 > 天然产物研究

	Ion-induced PCR strategy for mercury detection
	Chen, Gangyi 3; Wang, Wei 1,2,3; Yuan, Yi 3; Dong, Juan 3; Cui, Xin 3; Huang, Xin 3; Deng, Yun 1; Tang, Zhuo 3
刊名	ELECTROPHORESIS
	2020
卷号	41 页码:1517-1522
关键词	Fluorometric dye Ion-induced Mercury detection PCR amplification Thymine-Hg-thymine
ISSN号	0173-0835
DOI	10.1002/elps.201900324
产权排序	1
文献子类	Article
英文摘要	Mercury contamination is one of the most serious environmental problems. It can cause serious effects on the human health, such as case damage in the brain, nervous system, immune system, and kidney failure. Therefore, development of an accurate, sensitive, and simple operational detection method for mercury is very necessary. Herein, we report a new strategy for mercury ion detection based on commonly used PCR technique. High selectivity and sensitivity were achieved by the formation of the thymine-Hg-thymine (T-Hg-T) unnatural base pair at the 3'-end of PCR primers. The detection results of PCR amplification in presence of mercury ion could be reported either by using agarose gel analysis or through real-time fluorometric dye tracing for different detection purposes. To our knowledge, this study represents the first application of PCR based technique to the detection of metal ions.
学科主题	Chemistry & Analysis
URL标识	查看原文
WOS关键词	BASE-PAIRS ; HG-II ; DNA ; POLYMERASE ; SYSTEM
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	WILEY
WOS记录号	WOS:000502012100001
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30971]
专题	国家天然药物工程技术研究中心_天然产物研究
通讯作者	Tang, Zhuo
作者单位	1.Chengdu Univ TCM, State Key Lab Breeding Base Systemat Res Dev & Ut, Chengdu, Sichuan, Peoples R China 2.ChengDu Inst Food & Drug Control, Chengdu, Sichuan, Peoples R China; 3.Chinese Acad Sci, Nat Prod Res Ctr, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China;
推荐引用方式 GB/T 7714	Chen, Gangyi,Wang, Wei,Yuan, Yi,et al. Ion-induced PCR strategy for mercury detection[J]. ELECTROPHORESIS,2020,41:1517-1522.
APA	Chen, Gangyi.,Wang, Wei.,Yuan, Yi.,Dong, Juan.,Cui, Xin.,...&Tang, Zhuo.(2020).Ion-induced PCR strategy for mercury detection.ELECTROPHORESIS,41,1517-1522.
MLA	Chen, Gangyi,et al."Ion-induced PCR strategy for mercury detection".ELECTROPHORESIS 41(2020):1517-1522.