Evolution of microbial biosensor based on functional RNA through fluorescence-activated cell sorting

doi:10.1016/j.snb.2017.11.015

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 国家天然药物工程技术研究中心 > 天然产物研究

	Evolution of microbial biosensor based on functional RNA through fluorescence-activated cell sorting
	Li, Na; Huang, Xin; Zou, Jiawei; Chen, Gangyi; Liu, Getong; Li, Mei; Dong, Juan; Du, Feng; Cui, Xin; Tang, Zhuo
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2018
卷号	258 期号:2018 页码:550-557
关键词	Whole-cell Biosensor Aptamer RNA Spinach FACS
ISSN号	0925-4005
DOI	10.1016/j.snb.2017.11.015
产权排序	1
文献子类	Article
英文摘要	Whole-cell microbial biosensors are emerging as tools for rapid, economic and on-site detection. Anew approach to construct microbial biosensor with functional RNA for sensing of specific molecules is described here. The sensor is based on Spinach, an RNA mimic of GFP, and transcribed in living bacteria to detect intended molecules through fluorescent signals. Based on high-throughput cell cytometry, microbes with high selectivity and sensitivity could be screened out and evolved. Three microbial biosensors obtained through this method can efficiently detect tobramycin in different biosamples, providing a simple and economic solution for both qualitative and quantitative detection of antibiotics in the complex environments. This new strategy for the construction of whole-cell microbial biosensor and in vivo evolution protocol is especially suitable for any exogenous targets able to enter into bacteria and does not cause bacterial death in a short time, not just intracellular substances. (c) 2017 Elsevier B.V. All rights reserved.
学科主题	Spectroscopy ; Instrumentation ; Analytical Sciences
URL标识	查看原文
WOS关键词	TANDEM MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; AMINOGLYCOSIDE ANTIBIOTICS ; SYNTHETIC BIOLOGY ; RESIDUES ; APTAMER ; RIBOSWITCH ; BINDING ; PROTEIN ; TRANSLATION
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:000424868700063
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30736]
专题	国家天然药物工程技术研究中心_天然产物研究
作者单位	Univ Chinese Acad Sci, Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Li, Na,Huang, Xin,Zou, Jiawei,et al. Evolution of microbial biosensor based on functional RNA through fluorescence-activated cell sorting[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,258(2018):550-557.
APA	Li, Na.,Huang, Xin.,Zou, Jiawei.,Chen, Gangyi.,Liu, Getong.,...&Tang, Zhuo.(2018).Evolution of microbial biosensor based on functional RNA through fluorescence-activated cell sorting.SENSORS AND ACTUATORS B-CHEMICAL,258(2018),550-557.
MLA	Li, Na,et al."Evolution of microbial biosensor based on functional RNA through fluorescence-activated cell sorting".SENSORS AND ACTUATORS B-CHEMICAL 258.2018(2018):550-557.