A high-throughput in vivo selection method for luciferase variants

doi:10.1016/j.snb.2018.06.047

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 国家天然药物工程技术研究中心 > 天然产物研究

	A high-throughput in vivo selection method for luciferase variants
	Sun, Meiling 1,2; Fu, Zhicong 2; Wang, Tao 2; Cui, Xin 2; Dong, Juan 2; Du, Feng 2; Huang, Xin 2; Lu, Xue 2; Chen, Gangyi 2; Sun, Jian 2
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2018
卷号	273 期号:2018 页码:191-197
关键词	Gaussia luciferase Selection method Bioluminescence Optical sensor
ISSN号	0925-4005
DOI	10.1016/j.snb.2018.06.047
产权排序	1
文献子类	Article
英文摘要	Luciferase catalyzes its special substrate and light emits during the oxidation reaction, which is widely used as an optical sensor to monitor gene expression, protein-protein interaction and tumor response to therapy. However, limitations like the rapid decay, low stability and poor intensity of light emission make the optical signal hard to be captured in the sample detection. To obtain luciferase variants with improved features, traditional methods are usually based on CCD imaging technology or colony picking, which are low-throughput, time and labor consuming. Herein, we describe a novel luciferase screening method called fast library shrink method (FLSM), which could efficiently help select the desired mutant from the huge and complicated pool without analyzing the variants one by one. Based on FLSM, we successfully obtained one Gluc variant named M43IL119Q, which shows a 3-fold increase in the intensity of light emission as well as a stable light emission kinetics. The selection method is ready-to-use and cost-effective, thus making it a promising platform for the fast evolution of luciferases.
学科主题	Spectroscopy ; Instrumentation/analytical Sciences
URL标识	查看原文
WOS关键词	GAUSSIA ; EXPRESSION ; CELLS ; ASSAY
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:000441519000025
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30632]
专题	国家天然药物工程技术研究中心_天然产物研究
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Sichuan, Peoples R China;
推荐引用方式 GB/T 7714	Sun, Meiling,Fu, Zhicong,Wang, Tao,et al. A high-throughput in vivo selection method for luciferase variants[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,273(2018):191-197.
APA	Sun, Meiling.,Fu, Zhicong.,Wang, Tao.,Cui, Xin.,Dong, Juan.,...&Tang, Zhuo.(2018).A high-throughput in vivo selection method for luciferase variants.SENSORS AND ACTUATORS B-CHEMICAL,273(2018),191-197.
MLA	Sun, Meiling,et al."A high-throughput in vivo selection method for luciferase variants".SENSORS AND ACTUATORS B-CHEMICAL 273.2018(2018):191-197.