Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro

doi:10.1016/j.ceramint.2018.08.249

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 国家天然药物工程技术研究中心 > 天然产物研究

	Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro
	Ren, Xiaohua 7; Tuo, Qiang 6; Tian, Kun 7; Huang, Guo 5; Li, Jinyu 4; Xu, Taotao 4; Lv, Xinrong 3; Wu, Jia 2; Chen, Zhenghui 1; Weng, Jie 4
刊名	CERAMICS INTERNATIONAL
	2018
卷号	44 期号:17 页码:21656-21665
关键词	Bony defect Porous hydroxyapatite scaffolds Osteogenic
ISSN号	0272-8842
DOI	10.1016/j.ceramint.2018.08.249
产权排序	7
文献子类	Article
英文摘要	The repair of large maxillofacial bony defects using regular scaffolds is restricted by the osteogenic effect. It was postulated that a novel porous hydroxyapatite (HA) scaffolds with a 25-30 mu m groove structure (HAG) may counter this limitation. In this study we evaluated the biocompatibility of HAG scaffolds both in vitro and in vivo in beagle dogs by investigating the enhancement of scaffolds bioactivity and osteogenesis. Compared with a regular HA scaffolds, the HAG scaffolds significantly promoted human placenta-derived mesenchymal stem cell (hPMSC) osteogenic differentiation and the maturation of osteoblasts. This is achieved by increasing protein adsorption, as well as promoting directed growth and expression of osteogenic genes in vitro. The compressive strength of HAG scaffolds was significantly greater than HA in both dorsal muscle and the mandibular distraction area after in vivo implantation, with hematoxylin and eosin staining demonstrating new bone formation and vasculogenesis. Immunochemical staining and micro-CT scanning demonstrated increased expression of osteogenic factors (BMP2, OCN and COL-1) and bone density in the HAG scaffolds compared with HA. Based on the above results, we conclude that HAG scaffolds that have a groove structure induce greater osteogenesis and possess improved ostoegenesis which could be utilized in the clinical treatment.
学科主题	Applied Physics/condensed Matter/materials Science
URL标识	查看原文
WOS关键词	MESENCHYMAL STEM-CELLS ; BONE TISSUE ; BLOOD-VESSELS ; STROMAL CELLS ; PORE-SIZE ; REGENERATION ; SURFACE ; MODEL ; VASCULARIZATION ; OSTEOBLASTS
WOS研究方向	Materials Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000448226900135
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30618]
专题	国家天然药物工程技术研究中心_天然产物研究
作者单位	1.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2.Department of Stomatology, Nuclear Industry 416 Hospital, China; 3.Southwest Medical University School of Stomatolog, China; 4.Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu,Sichuan 610031,China; 5.The Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, School of Medicine,University of Electronic Science and Technology of China, China; 6.University of Electronic Science and Technology of China, China; 7.Department of Stomatology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, China;
推荐引用方式 GB/T 7714	Ren, Xiaohua,Tuo, Qiang,Tian, Kun,et al. Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro[J]. CERAMICS INTERNATIONAL,2018,44(17):21656-21665.
APA	Ren, Xiaohua.,Tuo, Qiang.,Tian, Kun.,Huang, Guo.,Li, Jinyu.,...&Mu, Yandong.(2018).Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro.CERAMICS INTERNATIONAL,44(17),21656-21665.
MLA	Ren, Xiaohua,et al."Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro".CERAMICS INTERNATIONAL 44.17(2018):21656-21665.