Profiling the origin, dynamics, and function of traction force in B cell activation

doi:10.1126/scisignal.aai9192

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 国家天然药物工程技术研究中心 > 天然产物研究

	Profiling the origin, dynamics, and function of traction force in B cell activation
	Wang, Junyi 10; Lin, Feng 9; Wan, Zhengpeng 10; Sun, Xiaolin 8; Lu, Yun 7; Huang, Jianyong 9; Wang, Fei 6; Zeng, Yingyue 5; Chen, Ying-Hua 10; Shi, Yan 4
刊名	SCIENCE SIGNALING
	2018
卷号	11 期号:542 页码:eaai9192
ISSN号	1945-0877
DOI	10.1126/scisignal.aai9192
产权排序	5
文献子类	Article
英文摘要	B lymphocytes use B cell receptors (BCRs) to recognize membrane-bound antigens to further initiate cell spreading and contraction responses during B cell activation. We combined traction force microscopy and live-cell imaging to profile the origin, dynamics, and function of traction force generation in these responses. We showed that B cell activation required the generation of 10 to 20 nN of traction force when encountering antigens presented by substrates with stiffness values from 0.5 to 1 kPa, which mimic the rigidity of antigen-presenting cells in vivo. Perturbation experiments revealed that F-actin remodeling and myosin- and dynein-mediated contractility contributed to traction force generation and B cell activation. Moreover, membrane-proximal BCR signaling molecules (including Lyn, Syk, Btk, PLC-gamma 2, BLNK, and Vav3) and adaptor molecules (Grb2, Cbl, and Dok-3) linking BCR microclusters and motor proteins were also required for the sustained generation of these traction forces. We found a positive correlation between the strength of the traction force and the mean fluorescence intensity of the BCR microclusters. Furthermore, we demonstrated that isotype-switched memory B cells expressing immunoglobulin G (IgG)-BCRs generated greater traction forces than did mature naive B cells expressing IgM-BCRs during B cell activation. Last, we observed that primary B cells from patients with rheumatoid arthritis generated greater traction forces than did B cells from healthy donors in response to antigen stimulation. Together, these data delineate the origin, dynamics, and function of traction force during B cell activation.
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WOS关键词	HIGH EPITOPE DENSITY ; MECHANICAL-PROPERTIES ; INTEGRIN ACTIVATION ; PROTEIN MOLECULE ; T-CELLS ; ADHESION ; ACTIN ; TAIL ; IGG ; STIFFNESS
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	AMER ASSOC ADVANCEMENT SCIENCE
WOS记录号	WOS:000440925500001
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30613]
专题	国家天然药物工程技术研究中心_天然产物研究
作者单位	1.Beijing Key Lab Immunol Res Chron Dis, Beijing 100084, Peoples R China 2.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China; 3.Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China.; 4.Tsinghua Univ, Ctr Life Sci, Inst Immunol, Dept Basic Med Sci, Beijing 100084, Peoples R China; 5.Liaoning Univ, Sch Life Sci, Shenyang 110036, Liaoning, Peoples R China; 6.Chinese Acad Sci, Chengdu Inst Biol, 9 Sect 4,Renmin South Rd, Chengdu 610041, Sichuan, Peoples R China; 7.Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China; 8.Peking Univ, Peoples Hosp, Dept Rheumatol & Immunol, Beijing 100871, Peoples R China; 9.Peking Univ, Coll Engn, Dept Mech & Engn Sci, Beijing 100871, Peoples R China; 10.Tsinghua Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, China Minist Educ, Key Lab Prot Sci,Sch Life Sci,Inst Immunol, Beijing 100084, Peoples R China;
推荐引用方式 GB/T 7714	Wang, Junyi,Lin, Feng,Wan, Zhengpeng,et al. Profiling the origin, dynamics, and function of traction force in B cell activation[J]. SCIENCE SIGNALING,2018,11(542):eaai9192.
APA	Wang, Junyi.,Lin, Feng.,Wan, Zhengpeng.,Sun, Xiaolin.,Lu, Yun.,...&Liu, Wanli.(2018).Profiling the origin, dynamics, and function of traction force in B cell activation.SCIENCE SIGNALING,11(542),eaai9192.
MLA	Wang, Junyi,et al."Profiling the origin, dynamics, and function of traction force in B cell activation".SCIENCE SIGNALING 11.542(2018):eaai9192.