PI(4,5)P2 determines the threshold of mechanical force-induced B cell activation

doi:10.1083/jcb.201711055

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	PI(4,5)P2 determines the threshold of mechanical force-induced B cell activation
	Wan, Zhengpeng 6; Xu, Chenguang 6; Chen, Xiangjun 6; Xie, Hengyi 6; Li, Zongyu 6; Wang, Jing 6; Ji, Xingyu 6; Chen, Haodong 5; Ji, Qinghua 4; Shaheen, Samina 6
刊名	JOURNAL OF CELL BIOLOGY
	2018-07-01
卷号	217 期号:7 页码:2565-2582
ISSN号	0021-9525
DOI	10.1083/jcb.201711055
产权排序	2
文献子类	Article
英文摘要	B lymphocytes use B cell receptors (BCRs) to sense the chemical and physical features of antigens. The activation of isotype-switched IgG-BCR by mechanical force exhibits a distinct sensitivity and threshold in comparison with IgM-BCR. However, molecular mechanisms governing these differences remain to be identified. In this study, we report that the low threshold of IgG-BCR activation by mechanical force is highly dependent on tethering of the cytoplasmic tail of the IgG-BCR heavy chain (IgG-tail)to the plasma membrane. Mechanistically, we show that the positively charged residues in the IgG-tail play a crucial role by highly enriching phosphatidylinositol (4,5)-biphosphate (PI(4,5)P2)into the membrane microdomains of IgG-BCRs. Indeed, manipulating the amounts of PI(4,5)P2 within IgG-BCR membrane microdomains significantly altered the threshold and sensitivity of IgG-BCR activation. Our results reveal a lipid-dependent mechanism for determining the threshold of IgG-BCR activation by mechanical force.
学科主题	Cell ; Developmental Biology
URL标识	查看原文
WOS关键词	HIGH EPITOPE DENSITY ; ANTIGEN RECEPTOR ; PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE ; SIGNALING MOLECULES ; PHOSPHATIDIC-ACID ; PROTEIN MOLECULE ; LIPID RAFTS ; SURFACE ; MEMORY ; ACTIN
WOS研究方向	Cell Biology
语种	英语
出版者	ROCKEFELLER UNIV PRESS
WOS记录号	WOS:000438081900028
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30217]
专题	国家天然药物工程技术研究中心_天然产物研究
作者单位	1.Beijing Key Lab Immunol Res Chron Dis, Beijing, Peoples R China 2.Zhejiang Univ, Sch Basic Med Sci, Hangzhou, Zhejiang, Peoples R China; 3.Univ Sci & Technol China, Sch Life Sci, Hefei, Anhui, Peoples R China; 4.Chinese Acad Sci, Lab RNA Biol, Inst Biophys, Beijing, Peoples R China; 5.Chinese Acad Sci, Chengdu Inst Biol, Natl Prod Res Ctr, Chengdu, Sichuan, Peoples R China; 6.Tsinghua Univ, Inst Immunol, Sch Life Sci,Minist Educ,Key Lab Prot Sci, Collaborat Innovat Ctr Diag & Treatment Infect Di, Beijing, Peoples R China;
推荐引用方式 GB/T 7714	Wan, Zhengpeng,Xu, Chenguang,Chen, Xiangjun,et al. PI(4,5)P2 determines the threshold of mechanical force-induced B cell activation[J]. JOURNAL OF CELL BIOLOGY,2018,217(7):2565-2582.
APA	Wan, Zhengpeng.,Xu, Chenguang.,Chen, Xiangjun.,Xie, Hengyi.,Li, Zongyu.,...&Liu, Wanli.(2018).PI(4,5)P2 determines the threshold of mechanical force-induced B cell activation.JOURNAL OF CELL BIOLOGY,217(7),2565-2582.
MLA	Wan, Zhengpeng,et al."PI(4,5)P2 determines the threshold of mechanical force-induced B cell activation".JOURNAL OF CELL BIOLOGY 217.7(2018):2565-2582.