Xinjiang herbal tea exerts immunomodulatory activity via TLR2/4-mediated MAPK signaling pathways in RAW264.7 cells and prevents cyclophosphamide-induced immunosuppression in mice

doi:10.1016/j.jep.2018.09.032

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 生物多样性与生态系统服务领域 > 中国科学院山地生态恢复与生物资源利用重点实验室

	Xinjiang herbal tea exerts immunomodulatory activity via TLR2/4-mediated MAPK signaling pathways in RAW264.7 cells and prevents cyclophosphamide-induced immunosuppression in mice
	Bai, Yujia 3,4; Jiang, Yunyao 5; Liu, Tingwu 4; Li, Fu 1; Zhang, Jianmei 3,4; Luo, Yanyan 4; Zhang, Liang 3,4; Yan, Guilong 4; Feng, Zuoshan 3; Li, Xueqin 2
刊名	JOURNAL OF ETHNOPHARMACOLOGY
	2019
卷号	228 页码:179-187
关键词	Xinjiang herbal tea Immunomodulatory effect Toll-like receptor Nuclear factor-kappa B
ISSN号	0378-8741
DOI	10.1016/j.jep.2018.09.032
产权排序	4
文献子类	Article
英文摘要	Ethnopharmacological relevance: A multi-herb Chinese medicinal formula consisting of a variety of medicinal and edible materials has long been consumed as a hot drink and immune enhancer for its efficiency to increase disease resistance in Xinjiang, China. However, no fundamental data has been collected associated with traditional consumption. The present work was designed to evaluate the immunostimulatory role of Xinjiang herbal tea (XMT-WE) in RAW 264.7 macrophages and cyclophosphamide (CTX)-induced immunosuppression mice model. Materials and methods: RAW 264.7 cells were treated with various concentrations of XMT-WE. Nitric oxide (NO) levels were determined using Griess reagents, and pro-inflammatory cytokines such as interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha were investigated with a cytometric bead array kit. The effects on mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and TNF-alpha were investigated. Furthermore, activation of nuclear factor (NF)-kappa B and AP-1 mitogen-activated protein kinase (MAPK) signaling pathways was investigated. Results: Pre-treatment with XMT-WE significantly increased secretion of NO, IL-6, and TNF-alpha. In addition, XMT-WE markedly increased expression of iNOS, COX-2, and TNF-alpha as well as AP-1 and NF-kappa B translocation from the cytoplasm into the nucleus, which was associated with an increase of phosphorylated ERK, JNK, and p38 as well as membrane receptors such as toll-like receptor (TLR) 2 and TLR4. Moreover, XMT-WE promoted the secretion of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) in cyclophosphamide (CTX)-induced immunosuppressive mice. Conclusion: These results indicated that XMT-WE at 50 mu g/ml exerts immunomodulatory activity via TLR2/4-mediated MAPK signaling pathways in RAW 264.7 cells. Furthermore, in vivo experiments revealed that XMT-WE at the dose of 50 and 100 mg/kg strongly stimulated inflammatory cytokines.
学科主题	Pharmacology & Toxicology
URL标识	查看原文
WOS关键词	IN-VITRO ; MACROPHAGE ; INFLAMMATION ; ACTIVATION ; MECHANISMS ; EXPRESSION ; BLOCKADE ; LEAVES ; AP-1
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000452344800019
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/31099]
专题	生物多样性与生态系统服务领域_中国科学院山地生态恢复与生物资源利用重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Mt Ecol Restorat & Bioresource Utilizat, Ecol Restorat Biodivers Conservat Key Lab Sichuan, Chengdu Inst Biol, Chengdu, Sichuan, Peoples R China; 2.Nanjing Med Univ, Affiliated Huaian Peoples Hosp 1, Dept Gerontol, Huanghe West Rd, Huaian 223300, Peoples R China 3.Xinjiang Agr Univ, Coll Food Sci & Pharm, Urumqi 830052, Peoples R China; 4.Huaiyin Normal Univ, Jiangsu Collaborat Innovat Ctr Reg Modern Agr & E, Jiangsu Key Lab Ecoagr Biotechnol Around Hongze L, Huaian 223300, Peoples R China; 5.China Acad Chinese Med Sci, Xiyuan Hosp, Beijing 100091, Peoples R China;
推荐引用方式 GB/T 7714	Bai, Yujia,Jiang, Yunyao,Liu, Tingwu,et al. Xinjiang herbal tea exerts immunomodulatory activity via TLR2/4-mediated MAPK signaling pathways in RAW264.7 cells and prevents cyclophosphamide-induced immunosuppression in mice[J]. JOURNAL OF ETHNOPHARMACOLOGY,2019,228:179-187.
APA	Bai, Yujia.,Jiang, Yunyao.,Liu, Tingwu.,Li, Fu.,Zhang, Jianmei.,...&Hu, Weicheng.(2019).Xinjiang herbal tea exerts immunomodulatory activity via TLR2/4-mediated MAPK signaling pathways in RAW264.7 cells and prevents cyclophosphamide-induced immunosuppression in mice.JOURNAL OF ETHNOPHARMACOLOGY,228,179-187.
MLA	Bai, Yujia,et al."Xinjiang herbal tea exerts immunomodulatory activity via TLR2/4-mediated MAPK signaling pathways in RAW264.7 cells and prevents cyclophosphamide-induced immunosuppression in mice".JOURNAL OF ETHNOPHARMACOLOGY 228(2019):179-187.