Molecular markers and cytogenetics to characterize a wheat-Dasypyrum villosum 3V (3D) substitution line conferring resistance to stripe rust

doi:10.1371/journal.pone.0202033

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 农业生物技术研究

	Molecular markers and cytogenetics to characterize a wheat-Dasypyrum villosum 3V (3D) substitution line conferring resistance to stripe rust
	Zhang, Jie 1,4; Jiang, Yun 4; Wang, Ying 4; Guo, Yuanlin 4; Long, Hai 2; Deng, Guangbing 2; Chen, Qian 4; Xuan, Pu 3
刊名	PLOS ONE
	2018
卷号	13 期号:8 页码:e0202033
ISSN号	1932-6203
DOI	10.1371/journal.pone.0202033
产权排序	3
文献子类	Article
英文摘要	Dasypyrum villosum has been used as a valuable gene resource for disease resistances, yield increase and quality improvement in wheat. A novel wheat-D. villosum alien introgression line CD-3 was generated through hybridization between the common wheat Chinese Spring (CS) and a CS-D. villosum 3V addition line having considerably high stripe rust resistance, which enable the characterization of a potential new stripe rust resistance gene (s) derived from D. villosum. The results of non-denaturing fluorescent in situ hybridization (ND-FISH) showed that CD-3 contained 42 chromosomes, including a 3V chromosome pair, and the absence of both of the 3D chromosomes. PCR-based Landmark Unique Gene (PLUG) molecular marker analysis supported results from the FISH analysis, revealing CD-3 was a wheat-D. villosum 3V (3D) disomic substitution line. Resistant test of stripe rust on 52 plants of F2 generation (CD-3/CS), CD-3, CS and D. villosum have been conducted at seedling stage. 7 plants of F2 generation possessing two 3V chromosomes exhibited high resistance to stripe rust as CD-3 and D. villosum, 10 plants carrying one 3V chromosome and 35 plants without 3V chromosome were susceptive to stripe rust as CS. The result implied the high stripe rust resistance of CD-3 should be controlled by recessive gene(s) originating from D. villosum. To rapidly detect chromosome 3V in the genetic background of wheat, we developed a novel Sequence Characterized Amplified Region (SCAR) marker specific for 3V chromosome based on the sequence of a grain size-related gene DvGS5 in D. villosum, an orthologue of TaGS5 from wheat. The SCAR marker was designated DvGS5-1443, which could successfully amplify a unique 3V-specific fragment in CD-3 and D. villosum, suggesting that this SCAR marker could facilitate targeting the chromosome 3V in the genetic background of wheat for wheat improvement.
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WOS关键词	POWDERY MILDEW RESISTANCE ; F-SP TRITICI ; CHROMOSOMAL-ABERRATIONS ; CEREAL EYESPOT ; GENUS SECALE ; HETEROCHROMATIN ; IDENTIFICATION ; REARRANGEMENTS ; SEQUENCES ; CHROMATIN
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000443071400028
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30604]
专题	环境治理与食品安全领域_农业生物技术研究
作者单位	1.Minist Agr, Key Lab Wheat Biol & Genet Improvement Southweste, Chengdu, Sichuan, Peoples R China; 2.Chinese Acad Sci, Chengdu Inst Biol, Chengdu, Sichuan, Peoples R China; 3.Sichuan Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Chengdu, Sichuan, Peoples R China 4.Sichuan Acad Agr Sci, Inst Biotechnol & Nucl Technol Res, Chengdu, Sichuan, Peoples R China;
推荐引用方式 GB/T 7714	Zhang, Jie,Jiang, Yun,Wang, Ying,et al. Molecular markers and cytogenetics to characterize a wheat-Dasypyrum villosum 3V (3D) substitution line conferring resistance to stripe rust[J]. PLOS ONE,2018,13(8):e0202033.
APA	Zhang, Jie.,Jiang, Yun.,Wang, Ying.,Guo, Yuanlin.,Long, Hai.,...&Xuan, Pu.(2018).Molecular markers and cytogenetics to characterize a wheat-Dasypyrum villosum 3V (3D) substitution line conferring resistance to stripe rust.PLOS ONE,13(8),e0202033.
MLA	Zhang, Jie,et al."Molecular markers and cytogenetics to characterize a wheat-Dasypyrum villosum 3V (3D) substitution line conferring resistance to stripe rust".PLOS ONE 13.8(2018):e0202033.