Stable-isotopic analysis and high-throughput pyrosequencing reveal the coupling process and bacteria in microaerobic and hypoxic methane oxidation coupled to denitrification

doi:10.1016/j.envpol.2019.04.111

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 应用与环境微生物研究

	Stable-isotopic analysis and high-throughput pyrosequencing reveal the coupling process and bacteria in microaerobic and hypoxic methane oxidation coupled to denitrification
	Cao, Qin 2; Liu, Xiaofeng 2; Li, Na 1; Xie, Zhijie 2; Li, Zhidong 2; Li, Dong 2
刊名	ENVIRONMENTAL POLLUTION
	2019
卷号	250 页码:863-872
关键词	Methane oxidation coupled to denitrification Stable isotope tracer Microbial diversity Methanotrophic bacteria Methylotrophic bacteria
ISSN号	0269-7491
DOI	10.1016/j.envpol.2019.04.111
产权排序	1
文献子类	Article
英文摘要	Microaerobic and hypoxic methane oxidation coupled to denitrification (MAME-D and HYME-D) occur in stabilized landfills with leachate recirculation when biological denitrification is limited by lack of organics. To evaluate nitrate denitrification efficiency and culture MAME-D/HYME-D involved bacteria, a leach bed bioreactor semi-continuous experiment was conducted for 60 days in 5 runs, under nitrate concentrations ranging of 20 mg/L-55 mg/L, wherein 5% sterile leachate was added during runs 4 and 5. Although the HYME-D system demonstrated high denitrification efficiency (74.93%) and nitrate removal rate reached 2.62 mmol N/(L.d), the MAME-D system exhibited a denitrification efficiency of almost 100% and nitrate removal rate of 4.37 mmol N/(L.d). The addition of sterile leachate increased the nitrate removal rate in both systems, but caused the decrease of methane consumption in HYME-D. A stable isotope batch experiment was carried out to investigate the metabolic products by monitoring the (CO2)-C-13 and (N2O)-N-15 production. The production of organic intermediates such as citrate, lactic acid, acetate, and propionic acid were also observed, which exhibited a higher yield in HYME-D. Variations in the microbial communities were analyzed during the semi-continuous experiment. MAME-D was mainly conducted by the association of type I methanotroph Methylomonas and the methylotrophic denitrifier Methylotenera. Methane fermentation processed by Methylomonas under hypoxic conditions produced more complex organic intermediates and increased the diversity of related heterotrophic denitrifiers. The addition of sterile real leachate, resulting in increase of COD/N, influenced the microbial community of HYME-D system significantly. (C) 2019 Published by Elsevier Ltd.
学科主题	Environment/ecology
URL标识	查看原文
WOS关键词	GENUS METHYLOMONAS ; SP NOV. ; WATER ; REMOVAL ; NITRATE
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000471088200092
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30489]
专题	环境治理与食品安全领域_应用与环境微生物研究
作者单位	1.Chinese Acad Sci, Chengdu Inst Biol, Key Lab Mt Ecol Restorat & Bioresource Utilizat, Ecol Restorat & Biodivers Conservat Key Lab Sichu, Chengdu 610041, Sichuan, Peoples R China 2.Chinese Acad Sci, Key Lab Environm & Appl Microbiol, Environm Microbiol Key Lab Sichuan Prov, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China;
推荐引用方式 GB/T 7714	Cao, Qin,Liu, Xiaofeng,Li, Na,et al. Stable-isotopic analysis and high-throughput pyrosequencing reveal the coupling process and bacteria in microaerobic and hypoxic methane oxidation coupled to denitrification[J]. ENVIRONMENTAL POLLUTION,2019,250:863-872.
APA	Cao, Qin,Liu, Xiaofeng,Li, Na,Xie, Zhijie,Li, Zhidong,&Li, Dong.(2019).Stable-isotopic analysis and high-throughput pyrosequencing reveal the coupling process and bacteria in microaerobic and hypoxic methane oxidation coupled to denitrification.ENVIRONMENTAL POLLUTION,250,863-872.
MLA	Cao, Qin,et al."Stable-isotopic analysis and high-throughput pyrosequencing reveal the coupling process and bacteria in microaerobic and hypoxic methane oxidation coupled to denitrification".ENVIRONMENTAL POLLUTION 250(2019):863-872.