Nitrogen addition reduces soil bacterial richness, while phosphorus addition alters community composition in an old-growth N-rich tropical forest in southern China

doi:10.1016/j.soilbio.2018.08.022

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 应用与环境微生物研究

	Nitrogen addition reduces soil bacterial richness, while phosphorus addition alters community composition in an old-growth N-rich tropical forest in southern China
	Wang, Hui 6; Liu, Shirong 6; Zhang, Xiao 3; Mao, Qinggong 4; Li, Xiangzhen 1; You, Yeming 5; Wang, Jingxin 2; Zheng, Mianhai 4; Zhang, Wei 4; Lu, Xiankai 4
刊名	SOIL BIOLOGY & BIOCHEMISTRY
	2018-12
卷号	127 期号:2018 页码:22-30
关键词	Nitrogen deposition Phosphorus addition Nitrogen-rich Soil bacterial diversity Soil bacterial community composition Tropical forest
ISSN号	0038-0717
DOI	10.1016/j.soilbio.2018.08.022
产权排序	4
文献子类	Article
英文摘要	Increased nitrogen (N) deposition endangers the biodiversity and stability of forest ecosystems, and much of the original phosphorus (P) parent material continues to decrease in most lowland tropical forests. It remains poorly understood as to how soil microbial diversity at a molecular level responds to the addition of excess N and mitigation of soil P limitation, as well as their influencing factors, in the N-rich tropical forest ecosystems. To reach a better understanding, we conducted a six-year N and P-addition experiment consisting of three treatments: N-addition (150 kg N ha(-1) yr(-1)), P-addition (150 kg P ha(-1) yr(-1)), and NP-addition (150 kg N ha(-1) yr(-1) plus 150 kg P ha(-1) yr(-1)), besides a control treatment in an old-growth tropical forest in southern China. We examined the snapshot responses of soil bacterial richness and community composition to the elevated N and P levels after six years using a 16S rRNA gene MiSeq sequencing method. The soil bacterial alpha-diversity, which is represented by Chao1 index in terms of bacterial richness, was 783 +/- 87 (mean +/- SD) across all samples in this study. The N addition caused a decline in soil bacterial richness, most likely through its negative effect on soil pH. The decrease in soil pH resulted from the direct N input and indirect NO3- increase. However, the P treatment had no effect on soil bacterial richness. The NP treatment also reduced the soil bacterial richness as the N addition. These results suggested that the P input could not alleviate the loss of soil bacterial richness induced by excess N deposition in the old-growth N-rich tropical forest. The Acidobacteria, which comprised 31.1% of the soil bacterial community, were the most dominant bacteria across all samples. The addition of P shifted the soil bacterial community composition. The elevated P availability with P-addition and the decreased understory plant coverage in the N-input treatment altered the soil bacterial beta-diversity. Our results highlight the different roles of N and P depositions in shaping the soil bacterial richness and community composition, thereby causing concomitant changes in understory plant and underground microbial communities in this ecosystem.
学科主题	Environment ; Ecology
URL标识	查看原文
WOS关键词	LONG-TERM NITROGEN ; MICROBIAL COMMUNITY ; PLANT DIVERSITY ; DEPOSITION ; LIMITATION ; UNDERSTORY ; ACIDIFICATION ; RESPIRATION ; RESPONSES ; ECOSYSTEM
WOS研究方向	Agriculture
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000449895600003
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30094]
专题	环境治理与食品安全领域_应用与环境微生物研究
作者单位	1.Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu 610041, Sichuan, Peoples R China; 2.West Virginia Univ, Div Forestry & Nat Resources, POB 6215, Morgantown, WV 26506 USA 3.Henan Univ, Coll Environm & Planning, Key Lab Geospotial Technol Middle & Lower Yellow, Kaifeng 475004, Henan, Peoples R China; 4.Chinese Acad Sci, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China; 5.Guangxi Univ, Coll Forestry, Nanning 530004, Guangxi, Peoples R China; 6.Chinese Acad Forestry, Inst Forest Ecol Environm & Protect, Chinas Natl Forestry & Grassland Adm, Key Lab Forest Ecol & Environm,Beijing 100091, Peoples R China;
推荐引用方式 GB/T 7714	Wang, Hui,Liu, Shirong,Zhang, Xiao,et al. Nitrogen addition reduces soil bacterial richness, while phosphorus addition alters community composition in an old-growth N-rich tropical forest in southern China[J]. SOIL BIOLOGY & BIOCHEMISTRY,2018,127(2018):22-30.
APA	Wang, Hui.,Liu, Shirong.,Zhang, Xiao.,Mao, Qinggong.,Li, Xiangzhen.,...&Mo, Jiangming.(2018).Nitrogen addition reduces soil bacterial richness, while phosphorus addition alters community composition in an old-growth N-rich tropical forest in southern China.SOIL BIOLOGY & BIOCHEMISTRY,127(2018),22-30.
MLA	Wang, Hui,et al."Nitrogen addition reduces soil bacterial richness, while phosphorus addition alters community composition in an old-growth N-rich tropical forest in southern China".SOIL BIOLOGY & BIOCHEMISTRY 127.2018(2018):22-30.