Salt-tolerant and - sensitive alfalfa (Medicago sativa) cultivars have large variations in defense responses to the lepidopteran insect Spodoptera litura under normal and salt stress condition

doi:10.1371/journal.pone.0181589

	Salt-tolerant and - sensitive alfalfa (Medicago sativa) cultivars have large variations in defense responses to the lepidopteran insect Spodoptera litura under normal and salt stress condition
	Lei, Yunting 1,2,3; Liu, Qing 3; Hettenhausen, Christian 1; Cao, Guoyan 1; Tan, Qing 1; Zhao, Weiye 1; Lin, Honghui 2; Wu, Jianqiang 1
刊名	PLOS ONE
	2017-07-18
卷号	12 期号:7 页码:e0181589
ISSN号	1932-6203
DOI	10.1371/journal.pone.0181589
产权排序	2
文献子类	Article
英文摘要	In nature, plants are often exposed to multiple stress factors at the same time. Yet, little is known about how plants modulate their physiology to counteract simultaneous abiotic and biotic stresses, such as soil salinity and insect herbivory. In this study, insect performance bioassays, phytohormone measurements, quantification of transcripts, and protein determination were employed to study the phenotypic variations of two alfalfa (Medicago sativa) cultivars in response to insect Spodoptera litura feeding under normal and salt stress condition. When being cultivated in normal soil, the salt-tolerant alfalfa cultivar Zhongmu-1 exhibited lower insect resistance than did the salt-sensitive cultivar Xinjiang Daye. Under salinity stress, the defense responses of Xinjiang Daye were repressed, whereas Zhongmu-1 did not show changes in resistance levels. It is likely that salinity influenced the resistance of Xinjiang Daye through suppressing the accumulation of jasmonic acid-isoleucine (JA-Ile), which is the bioactive hormone inducing herbivore defense responses, leading to attenuated trypsin proteinase inhibitor (TPI) activity. Furthermore, exogenous ABA supplementation suppressed the insect herbivory-induced JA/JA-Ile accumulation and levels of JAR1 (jasmonate resistant 1) and TPI, and further decreased the resistance of Xinjiang Daye, whereas Zhongmu-1 showed very little response to the increased ABA level. We propose a mechanism, in which high levels of abscisic acid induced by salt treatment may affect the expression levels of JAR1 and consequently decrease JA-Ile accumulation and thus partly suppress the defense of Xinjiang Daye against insects under salt stress. This study provides new insight into the mechanism by which alfalfa responds to concurrent abiotic and biotic stresses.
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WOS关键词	ABSCISIC-ACID ; SALINITY STRESS ; NICOTIANA-ATTENUATA ; INDUCED RESISTANCE ; ABIOTIC STRESSES ; GENE-EXPRESSION ; PLANTS ; HERBIVORES ; ARABIDOPSIS ; JASMONATE
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000406061400057
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/29896]
专题	成都生物研究所_生态研究
作者单位	1.Chinese Acad Sci, Dept Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming Inst Bot, Kunming, Yunnan, Peoples R China 2.Sichuan Univ, Minist Educ, Coll Life Sci, State Key Lab Hydraul & Mt River Engn, Key Lab Bio, Chengdu, Sichuan, Peoples R China; 3.Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China;
推荐引用方式 GB/T 7714	Lei, Yunting,Liu, Qing,Hettenhausen, Christian,et al. Salt-tolerant and - sensitive alfalfa (Medicago sativa) cultivars have large variations in defense responses to the lepidopteran insect Spodoptera litura under normal and salt stress condition[J]. PLOS ONE,2017,12(7):e0181589.
APA	Lei, Yunting.,Liu, Qing.,Hettenhausen, Christian.,Cao, Guoyan.,Tan, Qing.,...&Wu, Jianqiang.(2017).Salt-tolerant and - sensitive alfalfa (Medicago sativa) cultivars have large variations in defense responses to the lepidopteran insect Spodoptera litura under normal and salt stress condition.PLOS ONE,12(7),e0181589.
MLA	Lei, Yunting,et al."Salt-tolerant and - sensitive alfalfa (Medicago sativa) cultivars have large variations in defense responses to the lepidopteran insect Spodoptera litura under normal and salt stress condition".PLOS ONE 12.7(2017):e0181589.