Analysis of human serum phosphopeptidome by a focused database searching strategy

	Analysis of human serum phosphopeptidome by a focused database searching strategy
	Zhu, Jun 1; Wang, Fangjun 1; Cheng, Kai 1; Song, Chunxia 1; Qin, Hongqiang 1; Hu, Lianghai 2; Figeys, Daniel 3,4; Ye, Mingliang 1; Zou, Hanfa 1
刊名	journal of proteomics
	2013-01-14
卷号	78 页码:389-397
关键词	Human serum Phosphopeptidome Ti (IV)-MCM-41 Focused database
ISSN号	1874-3919
通讯作者	叶明亮 ; 邹汉法
产权排序	1,1
英文摘要	as human serum is an important source for early diagnosis of many serious diseases, analysis of serum proteome and peptidome has been extensively performed. however, the serum phosphopeptidome was less explored probably because the effective method for database searching is lacking. conventional database searching strategy always uses the whole proteome database, which is very time-consuming for phosphopeptidome search due to the huge searching space resulted from the high redundancy of the database and the setting of dynamic modifications during searching. in this work, a focused database searching strategy using an in-house collected human serum pro-peptidome target/decoy database (huspep) was established. it was found that the searching time was significantly decreased without compromising the identification sensitivity. by combining size-selective ti (iv)-mcm-41 enrichment, rp-rp off-line separation, and complementary cid and etd fragmentation with the new searching strategy, 143 unique endogenous phosphopeptides and 133 phosphorylation sites (109 novel sites) were identified from human serum with high reliability. (c) 2012 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	tandem mass-spectrometry ; capillary trap column ; rp-rp-hplc ; phosphoproteome analysis ; biomarker discovery ; enrichment ; peptidome ; identification ; extraction ; separation
收录类别	SCI
语种	英语
WOS记录号	WOS:000316706800030
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/117609]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Jilin Univ, Coll Life Sci, Changchun 130023, Peoples R China 3.Fac Med, Dept Biochem Microbiol & Immunol, Ottawa Inst Syst Biol, Ottawa, ON, Canada 4.Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada
推荐引用方式 GB/T 7714	Zhu, Jun,Wang, Fangjun,Cheng, Kai,et al. Analysis of human serum phosphopeptidome by a focused database searching strategy[J]. journal of proteomics,2013,78:389-397.
APA	Zhu, Jun.,Wang, Fangjun.,Cheng, Kai.,Song, Chunxia.,Qin, Hongqiang.,...&Zou, Hanfa.(2013).Analysis of human serum phosphopeptidome by a focused database searching strategy.journal of proteomics,78,389-397.
MLA	Zhu, Jun,et al."Analysis of human serum phosphopeptidome by a focused database searching strategy".journal of proteomics 78(2013):389-397.