The engineered biphenyl dioxygenases enhanced the metabolism of dibenzofuran

doi:10.1016/j.ibiod.2021.105228

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	The engineered biphenyl dioxygenases enhanced the metabolism of dibenzofuran
	Wang, Yuan 1,2,3; Sun, Chengcheng 2,3,5; Min, Jun 2,3,4; Li, Bingjun 1; Li, Junde 2,3,5; Chen, Weiwei 2,3,5; Kong, Yachao 2,3,5; Hu, Xiaoke 2,3,4
刊名	INTERNATIONAL BIODETERIORATION & BIODEGRADATION
	2021-07-01
卷号	161 页码:9
关键词	Dibenzofuran Burkholderia xenovorans LB400 Biphenyl dioxygenase Enzyme engineering Regiospecificity Biocatalysts
ISSN号	0964-8305
DOI	10.1016/j.ibiod.2021.105228
通讯作者	Hu, Xiaoke(xkhu@yic.ac.cn)
英文摘要	As a model compound of dioxin, dibenzofuran is a persistent environmental pollutant. Several investigations have provided evidence that biphenyl dioxygenase (BPDO) from Burkholderia xenovorans LB400 could be engineered to further enhance the metabolite profile of biphenyl and polychlorinated biphenyl through lateral oxygenation. In this context, we examined the ability of the evolved BphAES283M, BphAEp4-S283M and BphAERR41S283M to transform dibenzofuran with features of co-planar and ortho-substituted biphenyls. For BphAES283M, BphAEp4-S283M and BphAERR41-S283M, the kcat/Km value toward dibenzofuran was 4.5 times, 3 times and 2.5 times higher than that of the wild-type enzyme, respectively. Meanwhile, biochemical experiments determined that the substitution Ser283Met affected the regiospecificity of product formation, and the primary metabolite produced by BphAES283M was identified as 3,4-dihydro-3,4-dihydroxy-dibenzofuran. The structural analysis revealed residue Met283 as critical to generate a flexible catalytic cavity and a productive orientation of dibenzofuran during the catalytic reaction. Collectively, this study provides the theoretical basis and technical support for the significant development of better promising biocatalysts to effectively degrade dibenzofuran and other aromatic pollutants in the environment.
资助项目	National Natural Science Foundation of China[32070112] ; National Natural Science Foundation of China[32070102] ; Key Research Project of Frontier Science of Chinese Academy of Sciences[QYZDBSSWDQC041] ; External Cooperation Program of Chinese Academy of Sciences[133337KYSB20180015] ; Youth Innovation Promotion Association Program of the Chinese Academy of Sciences[2020218]
WOS关键词	ACTIVE-SITE ; NAPHTHALENE DIOXYGENASE ; BACTERIAL-DEGRADATION ; CATABOLIC PATHWAY ; P-DIOXIN ; STRAIN ; B-356 ; AMINO ; BURKHOLDERIA-XENOVORANS-LB400 ; DIBENZOTHIOPHENE
WOS研究方向	Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology
语种	英语
WOS记录号	WOS:000656677300004
资助机构	National Natural Science Foundation of China ; Key Research Project of Frontier Science of Chinese Academy of Sciences ; External Cooperation Program of Chinese Academy of Sciences ; Youth Innovation Promotion Association Program of the Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/29379]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Hu, Xiaoke
作者单位	1.Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Chunhui Rd, Yantai 264003, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yuan,Sun, Chengcheng,Min, Jun,et al. The engineered biphenyl dioxygenases enhanced the metabolism of dibenzofuran[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2021,161:9.
APA	Wang, Yuan.,Sun, Chengcheng.,Min, Jun.,Li, Bingjun.,Li, Junde.,...&Hu, Xiaoke.(2021).The engineered biphenyl dioxygenases enhanced the metabolism of dibenzofuran.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,161,9.
MLA	Wang, Yuan,et al."The engineered biphenyl dioxygenases enhanced the metabolism of dibenzofuran".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 161(2021):9.