Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses

doi:10.3389/fmars.2021.690879

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中国科学院牟平海岸带环境综合试验站

	Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses
	Han, Yijing 1,2,3; Hu, Gege 1; Chen, Yuying 4; Chen, Lizhu 4; Yu, Daode 5; Zhang, Qianqian 1,2; Yang, Dinglong 1,2
刊名	FRONTIERS IN MARINE SCIENCE
	2021-07-16
卷号	8 页码:10
关键词	Ruditapes philippinarum defensin extracellular traps antimicrobial effect membrane permeabilization
DOI	10.3389/fmars.2021.690879
通讯作者	Yu, Daode(yudaode@shandong.cn) ; Yang, Dinglong(dlyang@yic.ac.cn)
英文摘要	Although defensins have been isolated from a variety of metazoan, their role in cellular immunity has not been answered. In the study, we found that the hemocytes of the Manila clams Ruditapes philippinarum release defensin (designated as Rpdef3) in response to Vibrio parahaemolyticus challenge. The antimicrobial Rpdef3 was proved to be involved in the extracellular traps (ETs) that hemocytes released in response to Vibrio challenge. Scanning electron microscopy observation proved the patterns how ETs eliminate invading bacteria. Furthermore, Rpdef3 involved in ETs had broad-spectrum antimicrobial effect on both Gram-negative bacteria and Gram-positive bacteria. ELISA assay revealed that Rpdef3 could bind lipopolysaccharides and peptidoglycan in a dose-dependent manner. As concerned to the antibacterial mechanisms, Rpdef3 can cause bacterial membrane permeabilization, leading to cell death. As a result, Rpdef3 might contribute to the trap and the elimination of invading Vibrio in clam ETs. Taken together, our study suggest that the formation of ETs is a defense mechanism triggered by bacterial stimulation, coupled with antibacterial defensin.
资助项目	National Natural Science Foundation of China[41806196] ; Natural Science Foundation of Shandong Province[ZR2019BD022] ; Yantai Science and Technology Development Project[2020MSGY066] ; Youth Innovation Promotion Association of CAS[2019216]
WOS关键词	NEUTROPHIL EXTRACELLULAR TRAPS ; ESCHERICHIA-COLI ; ANTIBACTERIAL ACTIVITIES ; MEMBRANE ; PEPTIDES ; SHRIMP ; MECHANISMS ; HEMOCYTES ; JAPONICUS ; MOTILITY
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000680690300001
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project ; Youth Innovation Promotion Association of CAS
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/29527]
专题	中国科学院牟平海岸带环境综合试验站烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室海岸带生物资源高效利用研究与发展中心
通讯作者	Yu, Daode; Yang, Dinglong
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Shandong Marine Resource & Environm Res Inst, Yantai, Peoples R China 5.Marine Sci Res Inst Shandong Prov, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Han, Yijing,Hu, Gege,Chen, Yuying,et al. Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses[J]. FRONTIERS IN MARINE SCIENCE,2021,8:10.
APA	Han, Yijing.,Hu, Gege.,Chen, Yuying.,Chen, Lizhu.,Yu, Daode.,...&Yang, Dinglong.(2021).Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses.FRONTIERS IN MARINE SCIENCE,8,10.
MLA	Han, Yijing,et al."Antimicrobial Defensin and DNA Traps in Manila Clam Ruditapes philippinarum: Implications for Their Roles in Immune Responses".FRONTIERS IN MARINE SCIENCE 8(2021):10.