Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis

doi:10.1021/acssensors.1c00773

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis
	Gong, Fanwu 1; Wei, Hua-xing 2; Qi, Ji 3; Ma, Huan 1; Liu, Lianxin 4; Weng, Jianping 5; Zheng, Xucai 1; Li, Qiangsheng 1; Zhao, Dan 1; Fang, Haopeng 1
刊名	ACS SENSORS
	2021-07-23
卷号	6 期号:7 页码:2709-2719
关键词	SARS-Cov-2 COVID-19 diagnosis paper-based ELISA anti-RBD antibody serum separation microfluidic devices
ISSN号	2379-3694
DOI	10.1021/acssensors.1c00773
通讯作者	Jin, Tengchuan(jint@ustc.edu.cn) ; Li, Bowei(bwli@yic.ac.cn) ; Li, Bofeng(libf@ustc.edu.cn)
英文摘要	The spread of Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), resulting in a global pandemic with around four million deaths. Although there are a variety of nucleic acid-based tests for detecting SARS-CoV-2, these methods have a relatively high cost and require expensive supporting equipment. To overcome these limitations and improve the efficiency of SARS-CoV-2 diagnosis, we developed a microfluidic platform that collected serum by a pulling-force spinning top and paper-based microfluidic enzyme-linked immunosorbent assay (ELISA) for quantitative IgA/IgM/IgG measurements in an instrument-free way. We further validated the paper-based microfluidic ELISA analysis of SARS-CoV-2 receptor-binding domain (RBD)-specific IgA/IgM/IgG antibodies from human blood samples as a good measurement with higher sensitivity compared with traditional IgM/IgG detection (99.7% vs 95.6%) for early illness onset patients. In conclusion, we provide an alternative solution for the diagnosis of SARS-CoV-2 in a portable manner by this smart integration of pulling-force spinning top and paper-based microfluidic immunoassay.
资助项目	National Natural Science Foundation of China[41776110] ; National Natural Science Foundation of China[31870731] ; National Natural Science Foundation of China[81974258] ; COVID-19 special task grant from Chinese Academy of Science Clinical Research Hospital (Hefei)[YD2070002017] ; COVID-19 special task grant from Chinese Academy of Science Clinical Research Hospital (Hefei)[YD9110002006] ; Shandong Provincial Natural Science Foundation Key Project[ZR2020KB022] ; China Postdoctoral Science Foundation[2020M670084ZX]
WOS关键词	ANTIBODIES ; CHIP
WOS研究方向	Chemistry ; Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000677583700023
资助机构	National Natural Science Foundation of China ; COVID-19 special task grant from Chinese Academy of Science Clinical Research Hospital (Hefei) ; Shandong Provincial Natural Science Foundation Key Project ; China Postdoctoral Science Foundation
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/29597]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Jin, Tengchuan; Li, Bowei; Li, Bofeng
作者单位	1.Univ Sci & Technol China, Affiliated Hosp USTC 1, Dept Med Oncol, Div Life Sci & Med, Hefei 230001, Peoples R China 2.Univ Sci & Technol China, Div Life Sci & Med, Affiliated Hosp USTC 1, Dept Lab Med, Hefei 230001, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 4.Univ Sci & Technol China, Div Life Sci & Med, Affiliated Hosp USTC 1, Dept Hepatobiliary Surg, Hefei 230021, Peoples R China 5.Univ Sci & Technol China, Div Life Sci & Med, Affiliated Hosp USTC 1, Dept Endocrinol & Metab, Hefei 230027, Peoples R China 6.Yantai Univ, Sch Environm & Mat Engn, Yantai 264005, Peoples R China
推荐引用方式 GB/T 7714	Gong, Fanwu,Wei, Hua-xing,Qi, Ji,et al. Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis[J]. ACS SENSORS,2021,6(7):2709-2719.
APA	Gong, Fanwu.,Wei, Hua-xing.,Qi, Ji.,Ma, Huan.,Liu, Lianxin.,...&Li, Bofeng.(2021).Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis.ACS SENSORS,6(7),2709-2719.
MLA	Gong, Fanwu,et al."Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis".ACS SENSORS 6.7(2021):2709-2719.