Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy

doi:10.1021/acs.analchem.1c02386

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy
	Lu, Pengpeng 1,2; Zhang, Caiyun 1; Fu, Lili 1,2; Wei, Yinghui 2,3; Huang, Yan 1,2; Wang, Xiaoyan 1,2; Lv, Changjun 3; Chen, Lingxin 1,2,4
刊名	ANALYTICAL CHEMISTRY
	2021-07-27
卷号	93 期号:29 页码:10378-10387
ISSN号	0003-2700
DOI	10.1021/acs.analchem.1c02386
通讯作者	Huang, Yan(yhuang@yic.ac.cn) ; Wang, Xiaoyan(wangxy@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要	Pantetheinase (also known as Vanin-1) is highly expressed in the liver, kidneys, and intestine and is closely associated with a number of diseases. Vanin-1 can hydrolyze pantetheine to pantothenic acid (vitamin B5) and cysteamine and participate in the synthesis of glutathione (GSH). GSH is highly expressed in tumor cells and plays a major role in the resistance of tumor cells to cisplatin. Therefore, we urgently need a method to monitor the activity level of Vanin-1 in tumor cells and tissues and elucidate the relationship between the role of Vanin-1 in GSH synthesis and tumor resistance. Herein, we report a Cy-Pa fluorescent probe for imaging Vanin-1 in cells and in vivo that can qualitatively and quantitatively detect the fluctuation of Vanin-1 concentrations in HepG2 and HepG2/DDP cells or tumor tissues of tumor-bearing mice. This probe shows excellent potential in in situ real-time monitoring of endogenous Vanin-1. Moreover, we proved that Vanin-1 can inhibit GSH synthesis using the probe. When the Vanin-1 inhibitor RR6 was used in combination with cisplatin, HepG2 and HepG2/DDP cells showed increased resistance to cisplatin, while the therapeutic efficiency of cisplatin was reduced in HepG2 and HepG2/DDP xenografts. In this study, Vanin-1 was shown to play an important role in the treatment of cancer, and the study of Vanin-1 may provide an idea for the treatment of cancer in the future.
资助项目	National Nature Science Foundation of China[81573393] ; National Nature Science Foundation of China[21804010] ; National Nature Science Foundation of China[21976209] ; National Nature Science Foundation of China[22007005] ; Science and Technology Innovation Development Plan of Yantai of China[2020MSGY113] ; Science and Technology Innovation Development Plan of Yantai of China[2020YT06000898] ; Research Initiation Fund of Binzhou Medical University[BY2019KYQD39] ; Research Initiation Fund of Binzhou Medical University[BY2020KYQD01] ; Taishan Scholar Project Special Fund[ts20190962] ; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization[2018LS014]
WOS关键词	DRUG-RESISTANCE ; URINARY VANIN-1 ; CANCER ; GLUTATHIONE ; CISPLATIN ; PANTETHEINASE ; ASSAY ; CYSTEAMINE ; METABOLISM ; PATHWAY
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000679366000052
资助机构	National Nature Science Foundation of China ; Science and Technology Innovation Development Plan of Yantai of China ; Research Initiation Fund of Binzhou Medical University ; Taishan Scholar Project Special Fund ; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/29551]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Huang, Yan; Wang, Xiaoyan; Chen, Lingxin
作者单位	1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China 4.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Lu, Pengpeng,Zhang, Caiyun,Fu, Lili,et al. Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy[J]. ANALYTICAL CHEMISTRY,2021,93(29):10378-10387.
APA	Lu, Pengpeng.,Zhang, Caiyun.,Fu, Lili.,Wei, Yinghui.,Huang, Yan.,...&Chen, Lingxin.(2021).Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy.ANALYTICAL CHEMISTRY,93(29),10378-10387.
MLA	Lu, Pengpeng,et al."Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy".ANALYTICAL CHEMISTRY 93.29(2021):10378-10387.