Construction of a Polarity-Switchable Photoelectrochemical Biosensor for Ultrasensitive Detection of miRNA-141

doi:10.1021/acs.analchem.1c03460

	Construction of a Polarity-Switchable Photoelectrochemical Biosensor for Ultrasensitive Detection of miRNA-141
	Niu, Xiankang 1,2; Lu, Changtong 2; Su, Dan 1; Wang, Fang 2; Tan, Weihong 1; Qu, Fengli 1,2
刊名	ANALYTICAL CHEMISTRY
	2021-10-12
卷号	93
ISSN号	0003-2700
DOI	10.1021/acs.analchem.1c03460
通讯作者	Qu, Fengli(fengliquhn@hotmail.com)
英文摘要	As an early-stage tumor biomarker, microRNA (miRNA) has clinical application potential and its sensitive and accurate detection is significant for early tumor diagnosis. In this study, a photoelectrochemical (PEC) biosensing platform was fabricated for ultrasensitive miRNA-141 detection, which is based on a photocurrent polarity-switchable system using CdS quantum dots (QDs) in the presence of a 5,10,15,20-tetrakis (4-aminophenyl)-21H,23H-porphine (Tph-2H)-coated glassy carbon electrode (GCE). As an excellent photoactive material, Tph-2H has a narrow band gap that effectively gathers photoelectrons under visible light irradiation and improves the transfer ability of photogenerated electrons. Further, the detection sensitivity of miRNA-141 could be significantly improved by combining an enzyme-assisted recycle amplification reaction and a magnetic bead-based separation strategy. The proposed photocurrent polarity-switchable PEC biosensor could efficiently eliminate the false-positive or false-negative signals and achieve a wide linear response range from 1 fM to 1 nM with a low detection limit of 0.33 fM for miRNA-141, providing a potentially alternative solution for detecting other biomarkers in bioanalysis and clinical diagnosis.
资助项目	National Natural Science Foundation of China[21775089] ; National Natural Science Foundation of China[22074080] ; Changjiang Scholar Program of the Ministry of Education of China[Q2019258] ; Taishan Scholar Program of Shandong Province[tsqn201909106]
WOS关键词	MICRORNA ; ASSAY
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000708550500040
资助机构	National Natural Science Foundation of China ; Changjiang Scholar Program of the Ministry of Education of China ; Taishan Scholar Program of Shandong Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/125655]
专题	中国科学院合肥物质科学研究院
通讯作者	Qu, Fengli
作者单位	1.Chinese Acad Sci, Dept Pathol, Inst Basic Med & Canc, Canc Hosp,Univ Chinese Acad Sci, Hangzhou 310022, Zhejiang, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Niu, Xiankang,Lu, Changtong,Su, Dan,et al. Construction of a Polarity-Switchable Photoelectrochemical Biosensor for Ultrasensitive Detection of miRNA-141[J]. ANALYTICAL CHEMISTRY,2021,93.
APA	Niu, Xiankang,Lu, Changtong,Su, Dan,Wang, Fang,Tan, Weihong,&Qu, Fengli.(2021).Construction of a Polarity-Switchable Photoelectrochemical Biosensor for Ultrasensitive Detection of miRNA-141.ANALYTICAL CHEMISTRY,93.
MLA	Niu, Xiankang,et al."Construction of a Polarity-Switchable Photoelectrochemical Biosensor for Ultrasensitive Detection of miRNA-141".ANALYTICAL CHEMISTRY 93(2021).