Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis

doi:10.1093/toxres/tfab030

	Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis
	Shao, Chang-Sheng 2,3; Feng, Na 1,4; Zhou, Shuai 1; Zheng, Xin-Xin 2,3; Wang, Peng 2; Zhang, Jing-Song 1,4; Huang, Qing 2,3
刊名	TOXICOLOGY RESEARCH
	2021-05-01
卷号	10
关键词	ganoderic acid T (GAT) triterpene apoptosis necroptosis radiosensitivity ionizing radiation
ISSN号	2045-452X
DOI	10.1093/toxres/tfab030
通讯作者	Zhang, Jing-Song(syja16@saas.sh.cn) ; Huang, Qing(huangq@ipp.ac.cn)
英文摘要	The use of natural substances derived from traditional Chinese medicine and natural plants as safe radiosensitizing adjuvants is a new trend for cancer radiotherapy. Ganoderma lucidum has been used as a traditional Chinese medicine with a history of more than 2000 years. Ganoderic acid T (GAT) is a typical triterpene of G. lucidum, which has strong cytotoxicity to cancer cells, but whether it has radiation sensitization effect has not been explored. In this work, we treated the HeLa cells with different concentrations of GAT before exposure to gamma-ray radiation and investigated its influence on the radiosensitivity. The cell viability, apoptosis rate, necoptosis rate, intracellular ATP level, cell cycle, the amount of H2AX and 53BP1, reactive oxygen species, and mitochondrial membrane potential were examined. Apoptotic, necroptotic, and autophagic biomarker proteins, including caspase 8, cytochrome c, caspase 3, RIPK, MLKL, P62, and LC3, were analyzed. As a result, we confirmed that with treatment of GAT, the gamma-ray radiation induced both apoptosis and necroptosis in HeLa cells, and with increase of GAT, the percentage ratio of necroptosis was increased. The involved pathways and mechanisms were also explored and discussed.
资助项目	National Natural Science Foundation of China[11635013] ; National Natural Science Foundation of China[11775272] ; Central-level scientific institutions[Y79XG13361]
WOS关键词	CANCER-CELLS ; DEATH FATE ; INHIBITION ; LUCIDUM ; SWITCH
WOS研究方向	Toxicology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000671151100018
资助机构	National Natural Science Foundation of China ; Central-level scientific institutions
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/123505]
专题	中国科学院合肥物质科学研究院
通讯作者	Zhang, Jing-Song; Huang, Qing
作者单位	1.Minist Agr, Key Lab Edible Fungi Resources & Utilizat South, Beijing, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, CAS Key Lab High Magnet Field & Ion Beam Phys Bio, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Hefei 230026, Peoples R China 4.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Inst Edible Fungi, Shanghai 201403, Peoples R China
推荐引用方式 GB/T 7714	Shao, Chang-Sheng,Feng, Na,Zhou, Shuai,et al. Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis[J]. TOXICOLOGY RESEARCH,2021,10.
APA	Shao, Chang-Sheng.,Feng, Na.,Zhou, Shuai.,Zheng, Xin-Xin.,Wang, Peng.,...&Huang, Qing.(2021).Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis.TOXICOLOGY RESEARCH,10.
MLA	Shao, Chang-Sheng,et al."Ganoderic acid T improves the radiosensitivity of HeLa cells via converting apoptosis to necroptosis".TOXICOLOGY RESEARCH 10(2021).