In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic Neisseria gonorrhoeae

doi:10.1080/21655979.2021.1955548

	In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic Neisseria gonorrhoeae
	Liu, Jia 2; Yang, Chunjun 2; Cheng, Cheng 1; Zhang, Chenchen 2; Zhao, Jun 2; Fu, Chuyu 2
刊名	BIOENGINEERED
	2021
卷号	12
关键词	Neisseria gonorrhoeae plasma-activated liquid biocidal efficacy antimicrobial mechanism inflammation
ISSN号	2165-5979
DOI	10.1080/21655979.2021.1955548
通讯作者	Yang, Chunjun(yangchunjun9@163.com)
英文摘要	Neisseria gonorrhoeae (Ng) is highly resistant to treatment, and there is an urgent need for new treatments to alleviate gonococcal resistance caused by antibiotic monotherapy. The antimicrobial effect and mechanism of plasma-activated liquid (PAL) on Ng were evaluated in this study. Upon PAL treatment, extensively analyses on cell culturability, metabolic capacity, intracellular reactive oxygen species (ROS),membrane integrity and nucleic acids for Ng were carried out and significant antimicrobial effects observed.PAL exerted antibacterial effect on Ng and induced bacterial death (6.71-log) following immersion for 30 min and treatment for 120 s. However, bacterial viability test revealed that after immersion in the same PAL, 10.17% of bacteria retained their metabolic capacity. This indicates that bacteria enter a physiological viable but non-culturable state to protect themselves from environmental stress. Confocal fluorescence microscopy and transmission electron microscopy demonstrated that PAL exerts bactericidal effect on Ng and disrupts its morphological structure. PAL may upregulate inflammatory factors and genes to modulate the resistance of Ng and affect the immune status of the host during infection.
资助项目	Clinical Research Cultivation Program of The Second Affiliated Hospital of Anhui Medical University[2020LCZD21] ; Natural Science Research Project of Anhui Universities[KJ2020ZD19] ; National Natural Science Foundation of China[51777206]
WOS关键词	ESCHERICHIA-COLI ; STAPHYLOCOCCUS-AUREUS ; SPOILAGE CAPABILITY ; LIPID-PEROXIDATION ; INACTIVATION ; RESISTANCE ; NLRP3 ; STATE ; WATER
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000679091200001
资助机构	Clinical Research Cultivation Program of The Second Affiliated Hospital of Anhui Medical University ; Natural Science Research Project of Anhui Universities ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/123149]
专题	中国科学院合肥物质科学研究院
通讯作者	Yang, Chunjun
作者单位	1.Chinese Acad Sci, Inst Plasma Phys, Hefei, Peoples R China 2.Anhui Med Univ, Affiliated Hosp 2, Dept Dermatol, Hefei 230601, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jia,Yang, Chunjun,Cheng, Cheng,et al. In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic Neisseria gonorrhoeae[J]. BIOENGINEERED,2021,12.
APA	Liu, Jia,Yang, Chunjun,Cheng, Cheng,Zhang, Chenchen,Zhao, Jun,&Fu, Chuyu.(2021).In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic Neisseria gonorrhoeae.BIOENGINEERED,12.
MLA	Liu, Jia,et al."In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic Neisseria gonorrhoeae".BIOENGINEERED 12(2021).