Real-time tracking of lipid droplets interactions with other organelles by a high signal/noise probe

doi:10.1016/j.dyepig.2021.109366

	Real-time tracking of lipid droplets interactions with other organelles by a high signal/noise probe
	Ren, Wei 1,2; Wang, Dong 1,2; Huang, Wei 1,2; Li, Jiajia 1,2; Tian, Xiaohe 1,2; Liu, Zhengjie 1,2; Han, Guangmei 1,2; Liu, Bianhua 4; Han, Ming-Yong 4; Zhang, Zhongping 1,2,3
刊名	DYES AND PIGMENTS
	2021-07-01
卷号	191
关键词	Hydrophobic probe High signal/noise Lipid droplets Organelles interaction
ISSN号	0143-7208
DOI	10.1016/j.dyepig.2021.109366
通讯作者	Zhang, Ruilong(zrl@ahu.edu.cn)
英文摘要	Investigating the interactions between lipid droplets (LDs) and other organelles are greatly significant to the indepth understand the physiology and pathology of LDs. Despite of commercial LDs probes available, the ER and other membrane structures are also stained due to their amphiphilicity, which limits their further applications. To address this issue, we herein report a highly hydrophobic fluorescent probe to specifically label intracellular LDs with high signal/noise ratio, avoiding undesirable staining of ER and thus achieving to real-time monitor the interactions between LDs and other organelles. The probe showed strong emission in lipid environments but no fluorescence in water and liposomes, enabling the probe with high signal/noise ratio. Using this probe, it was found that the contacts of LDs with ER and mitochondria clearly increased under the condition of starvation.
资助项目	National Natural Science Foundation of China[21775001] ; National Natural Science Foundation of China[21874137] ; National Natural Science Foundation of China[21974001] ; National Natural Science Foundation of China[21976183] ; National Natural Science Foundation of China[22074001] ; Anhui Provincial Natural Science Foundation of China[1808085MB32]
WOS关键词	FLUORESCENT-PROBES ; METABOLISM ; RAB18 ; ER
WOS研究方向	Chemistry ; Engineering ; Materials Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000652615400004
资助机构	National Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/122608]
专题	中国科学院合肥物质科学研究院
通讯作者	Zhang, Ruilong
作者单位	1.Anhui Univ, Sch Chem & Chem Engn, Hefei 230601, Peoples R China 2.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Peoples R China 3.Anhui Univ, Minist Educ, Key Lab Struct & Funct Regulat Hybrid Mat, Hefei 230601, Anhui, Peoples R China 4.Chinese Acad Sci, Inst Solid State Phys, Key Lab Photovolta & Energy Conservat Mat, HFIPS, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Ren, Wei,Wang, Dong,Huang, Wei,et al. Real-time tracking of lipid droplets interactions with other organelles by a high signal/noise probe[J]. DYES AND PIGMENTS,2021,191.
APA	Ren, Wei.,Wang, Dong.,Huang, Wei.,Li, Jiajia.,Tian, Xiaohe.,...&Zhang, Ruilong.(2021).Real-time tracking of lipid droplets interactions with other organelles by a high signal/noise probe.DYES AND PIGMENTS,191.
MLA	Ren, Wei,et al."Real-time tracking of lipid droplets interactions with other organelles by a high signal/noise probe".DYES AND PIGMENTS 191(2021).