Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces

doi:10.1038/s41598-021-81816-1

	Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces
	Almeida, Luis C.2; Frade, Tania 2; Correia, Rui D.2; Niu Y(牛宇)1; Jin G(靳刚)1; Correia, Jorge P.2; Viana, Ana S.2
刊名	SCIENTIFIC REPORTS
	2021-01-26
卷号	11 期号:1 页码:12
ISSN号	2045-2322
DOI	10.1038/s41598-021-81816-1
通讯作者	Viana, Ana S.(anaviana@fc.ul.pt)
英文摘要	We report a straightforward and reproducible electrochemical approach to develop polydopamine-ethanolamine (ePDA-ETA) films to be used as immunosensing interfaces. ETA is strongly attached to polydopamine films during the potentiodynamic electropolymerization of dopamine. The great advantage of the electrochemical methods is to generate the oxidized species (quinones), which can readily react with ETA amine groups present in solution, with the subsequent incorporation of this molecule in the polymer. The presence of ETA and its effect on the electrosynthesis of polydopamine was accessed by cyclic voltammetry, ellipsometry, atomic force microscopy, FTIR and X-ray photoelectron spectroscopy. The adhesive and biocompatible films enable a facile protein linkage, are resilient to flow assays, and display intrinsic anti-fouling properties to block non-specific protein interactions, as monitored by real-time surface plasmon resonance, and confirmed by ellipsometry. Immunoglobulin G (IgG) and Anti-IgG were used in this work as model proteins for the affinity sensor. By using the one-step methodology (ePDA-ETA), the lower amount of immobilized biorecognition element, IgG, compared to that deposited on ePDA or on ETA post-modified film (ePDA/ETA), allied to the presence of ETA, improved the antibody-antigen affinity interaction. The great potential of the developed platform is its versatility to be used with any target biorecognition molecules, allowing both optical and electrochemical detection.
分类号	二类/Q1
资助项目	Fundacao para a Ciencia e Tecnologia (FCT)[UIDB/00100/2020] ; Fundacao para a Ciencia e Tecnologia (FCT)[SFRH/BD/129566/2017] ; Fundacao para a Ciencia e Tecnologia (FCT)[PTDC/CTM-NAN/0994/2014]
WOS关键词	SURFACE-CHEMISTRY ; CARBON-DISULFIDE ; DOPAMINE ; ADSORPTION ; LACCASE ; IMMOBILIZATION ; NANOPARTICLES ; OXIDATION ; OXIDE ; TOOL
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000667507900024
资助机构	Fundacao para a Ciencia e Tecnologia (FCT)
其他责任者	Viana, Ana S.
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/87031]
专题	力学研究所_国家微重力实验室
作者单位	1.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, NML, Beijing 100190, Peoples R China 2.Univ Lisbon, Fac Ciencias, Ctr Quim Estrutural, P-1749016 Lisbon, Portugal;
推荐引用方式 GB/T 7714	Almeida, Luis C.,Frade, Tania,Correia, Rui D.,et al. Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces[J]. SCIENTIFIC REPORTS,2021,11(1):12.
APA	Almeida, Luis C..,Frade, Tania.,Correia, Rui D..,牛宇.,靳刚.,...&Viana, Ana S..(2021).Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces.SCIENTIFIC REPORTS,11(1),12.
MLA	Almeida, Luis C.,et al."Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces".SCIENTIFIC REPORTS 11.1(2021):12.