Application of the Ludox-QPS method for estimating ciliate diversity in soil and comparison with direct count and DNA fingerprinting

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生物分类与系统演化实验室

	Application of the Ludox-QPS method for estimating ciliate diversity in soil and comparison with direct count and DNA fingerprinting
	Zhao, Feng 1,2; Xu, Kuidong1 ; He, Yingying 1,2
刊名	EUROPEAN JOURNAL OF SOIL BIOLOGY
	2012-03-01
卷号	49 页码:112-118
关键词	Bioindicator DGGE Direct count method Ludox density gradient centrifugation Sequencing
ISSN号	1164-5563
通讯作者	Xu, KD (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Soil ciliates have advantages in monitoring soil quality after pollution and disturbance. However the potential is limited by the shortcoming of integrating quantitative and qualitative techniques. Here we utilized the Ludox-Quantitative Protargol Stain (Ludox-QPS) method which was originally developed for marine sediment to assess ciliate diversity in soil. The method was tested with three soil samples and compared with a ciliate-specific Denaturing Gradient Gel Electrophoresis (DGGE) and direct count method. The Ludox-QPS method obtained significantly higher number of ciliates with higher taxonomic resolution than the direct count, and revealed higher diversity than the DGGE as well as subsequent gene sequencing. Besides, we evaluated the effects of 2% glutaraldehyde fixative and the QPS preparation on cell size of one species from each of the three dominant ciliate assemblages in soil: colpodids, hypotrichs and haptorids. The experiment indicates a biovolume conversion factor of similar to 0.69 for soil ciliates treated with the Ludox-QPS method. The Ludox-QPS method fulfills the enumeration of soil ciliates with good taxonomic resolution and thus promotes the utilization of ciliates in the bioassessment of soil environments. (C) 2011 Elsevier Masson SAS. All rights reserved.
学科主题	Ecology ; Soil Science
收录类别	SCI
原文出处	10.1016/j.ejsobi.2011.11.002
语种	英语
WOS记录号	WOS:000302736500016
公开日期	2013-09-25
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12525]
专题	海洋研究所_海洋生物分类与系统演化实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Feng,Xu, Kuidong,He, Yingying. Application of the Ludox-QPS method for estimating ciliate diversity in soil and comparison with direct count and DNA fingerprinting[J]. EUROPEAN JOURNAL OF SOIL BIOLOGY,2012,49:112-118.
APA	Zhao, Feng,Xu, Kuidong,&He, Yingying.(2012).Application of the Ludox-QPS method for estimating ciliate diversity in soil and comparison with direct count and DNA fingerprinting.EUROPEAN JOURNAL OF SOIL BIOLOGY,49,112-118.
MLA	Zhao, Feng,et al."Application of the Ludox-QPS method for estimating ciliate diversity in soil and comparison with direct count and DNA fingerprinting".EUROPEAN JOURNAL OF SOIL BIOLOGY 49(2012):112-118.