Cleaning of Marine Sediment Samples for Large Diatom Stable Isotope Analysis

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋地质与环境重点实验室

	Cleaning of Marine Sediment Samples for Large Diatom Stable Isotope Analysis
	Xiong, Zhifang 1; Li, Tiegang1 ; Crosta, Xavier 2
刊名	JOURNAL OF EARTH SCIENCE
	2012-04-01
卷号	23 期号:2 页码:161-172
关键词	large diatom stable isotope physical separation chemical oxidation Parece Vela basin palaeoceanography
ISSN号	1674-487X
通讯作者	Li, TG (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China.
英文摘要	Diatom stable isotope analysis offers considerable potential in palaeoceanography, particularly where carbonate material is scarce or absent. However, extracting pure diatom frustules free of external labile organic matter from marine sediments is an essential requirement for their applications as paleoenvironmental proxies. Here, based largely on previous work, we developed a method including physical separation and chemical oxidation steps to concentrate and clean pure large diatoms from laminated diatom mat and diatomaceous clay sediment samples for their stable isotope analysis. Using the physical separation techniques consisting of the removal of carbonate and excess organic matter, sieving, differential settling, and heavy liquid floatation, pure diatoms can be successfully isolated from the sediment samples with opal concentration more than 10%. Subsequent time oxidation experiment shows that labile organic matter coating pure diatom valves can be effectively removed with 30% H2O2 at 65 degrees C for 2 h. Measurements of delta C-13 after every step of physical separation demonstrate that contaminants and lost diatoms can influence the original diatom stable isotope signal, highlighting the importance of a visual check for dominant diatom size in the initial sample and purity in the final sample. Although the protocol described here was only applied to diatom mats or diatom oozes containing large diatoms (Ethmodiscus rex), we believe that this method can be adapted to common diatoms of general marine sediment samples.
学科主题	Geosciences, Multidisciplinary
收录类别	SCI
原文出处	10.1007/s12583-012-0241-x
语种	英语
WOS记录号	WOS:000302526200004
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12494]
专题	海洋研究所_海洋地质与环境重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China 2.Univ Bordeaux 1, UMR CNRS EPOC 5805, F-33405 Talence, France
推荐引用方式 GB/T 7714	Xiong, Zhifang,Li, Tiegang,Crosta, Xavier. Cleaning of Marine Sediment Samples for Large Diatom Stable Isotope Analysis[J]. JOURNAL OF EARTH SCIENCE,2012,23(2):161-172.
APA	Xiong, Zhifang,Li, Tiegang,&Crosta, Xavier.(2012).Cleaning of Marine Sediment Samples for Large Diatom Stable Isotope Analysis.JOURNAL OF EARTH SCIENCE,23(2),161-172.
MLA	Xiong, Zhifang,et al."Cleaning of Marine Sediment Samples for Large Diatom Stable Isotope Analysis".JOURNAL OF EARTH SCIENCE 23.2(2012):161-172.