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题名	气升式环流反应器中氧化亚铁硫杆菌的培养特性
作者	罗林
学位类别	硕士
答辩日期	2001-06
授予单位	中国科学院研究生院
导师	蔡昭铃
关键词	氧化亚铁硫杆菌 气升式反应器 生长特性 生物浸出 Fe~(2+)氧化速率
其他题名	The Cultural Characteristics of Thiobacillus Ferrooxidans in the Airlift Loop Reactor
学位专业	生化工程
中文摘要	氧化亚铁硫杆菌是生物冶金过程中应用最为广泛的浸矿微生物.该文首先在摇瓶水平上，实验研究了氧化亚铁硫杆菌的培养条件，并对培养温度、pH、起始Fe<'2+>浓度等主要因素进行了优化.基于生物浸出体系的物系特性，构建了一套在线检测系统较完备的气体提升式反应器，对反应芧238L髦械牧魈宓难妨鞫俣取⒒旌鲜奔洹⑵禾寤醮氏凳戎饕魈辶ρ形辛耸笛檠芯?在此基础上，较系统研究了氧化亚铁硫杆菌在气升式反应器中的培养特性.最后，在气升式反应器中进行了低品位镍铜硫化矿的生物治安出的探索实验，取得了较好的结果.
英文摘要	Bioleaching process of mental ores has a unique advantage in extraction of valuable metals from low-grade complex ores and is a prospective technology for its low cost, high recovery of metal and low environmental pollution. In most bioleaching processes, the growth of microorganisms is the limiting step. So the study of the growth of the bacteria in the reactor and the optimization of cultivation conditions are important to enhance the leaching rate. Thiobacillus ferrooxidans are the most widely used microorganisms in bioleacing process. In this work, the cultivation conditions of Thiobacillus ferrooxidans were studied in flask bottles and the major factors including culture temperature, pH and initial Fe~(2+) concentration were optimized. The optimal cultural conditions with Leathen medium are at 30℃, initial pH 2.5 and initial Fe~(2+) concentration 6g/L. But 30℃, initial pH 2.5 and initial Fe~(2+) concentration l0g/L are the optimal conditions with 9K medium. According to the properties of the bioleaching systems, an airlift reactor with a complete on-line measuring system was constructed and the major liquid dynamics including liquid circulation rate, liquid mixing time and volumetric oxygen mass transfer coefficient k_La were measured. The experimental results showed that liquid circulation rate, liquid mixing time, gas hold-up and gas-liquid mass transfer coefficient depended strongly on gas superficial velocity in the airlift reactor. In proper range, the increase of gas velocity can improve liquid circulation, mixing and mass transfer in the reactor. Based on above experimental results, the characteristics of the culture of Thiobacillus ferrooxidans in the airlift reactor was studied. The Leathen and 9K medium were used in the experiments. The growth of Thiobacillus ferrooxidans, Fe~(2+) oxidation rate, and changes of dissolved oxygen under different conditions were measured, from which the cultivation conditions of Thiobacillus ferrooxidans were preliminary optimized. The results indicated that the airlift reactor was suitable for the cultivation of Thiobacillus ferrooxidans and the growth of the bacteria was corresponding to dissolved oxygen and Fe~(2+) oxidation rate during the culture process. The 9K medium due to higher nitrogen is better for the growth of Thiobacillus ferrooxidans than Leathen medium. Fe~(2+) oxidation rate is related to initial Fe~(2+) oncentration and properly enhancing initial Fe~(2+) concentration can promote Fe~(2+) oxidation rate. Enlarging air rate to keep dissolved oxygen above 40% is necessary to the growth of bacteria during the cultivation. In the end of this work, the bioleaching of low-grade nickel-copper sulfide ores in the airlift reactor was carried out and preliminary results were obtained. The leaching fractions of nickel and copper at 15% pulp concentration reached 92.47% and 39.2% respectively in ten days.
语种	中文
公开日期	2013-09-26
页码	83
内容类型	学位论文
源URL	[http://ir.ipe.ac.cn/handle/122111/1898]
专题	过程工程研究所_研究所（批量导入）
推荐引用方式 GB/T 7714	罗林. 气升式环流反应器中氧化亚铁硫杆菌的培养特性[D]. 中国科学院研究生院. 2001.

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