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题名	蛋白质溶液微滤过程中膜污染机理的研究
作者	张建东
学位类别	博士
答辩日期	2003
授予单位	中国科学院过程工程研究所
授予地点	中国科学院过程工程研究所
导师	欧阳藩
关键词	微滤 蛋白质 膜污染 膜分离 微滤滤速
其他题名	Studies on Mechanisms of Membrane Fouling in Microfiltration of Protein Solutions
学位专业	化学工艺
中文摘要	蛋白质对微滤膜的污染是限制微滤技术在生物工程领域中广泛应用的一个主要因素.该文就微滤过程中引起膜污染的主要原因、溶液中蛋白质分子聚集体(aggregates,以下简称聚集体)的形成以及聚集体浓度对微滤速度的影响等方面,对微滤过程中膜污染现象及其机理进行了系统的研究.
英文摘要	Protein fouling of membranes is an obstacle to the wide application of microfiltration in biotechnology. This thesis investigated three aspects of protein fouling of membranes, including the cause of membrane fouling, the formation of aggregate in solutions, and the effect of concentration of aggregates on flux of microfiltration. A novel method for determining the amount of proteins on membranes was developed, which was precise, stable and repeatable. The method can be applied to commonly used membranes such as mixed esters of cellulose nitrate and acetate membranes, Durapore GVWP membranes, and track-etched polycarbonate membranes. Adsorption and deposition of proteins on membranes was observed under both static and filtrating conditions. It was shown that adsorption and deposition of proteins on membranes occurred simultaneously in microfiltration. Adsorption of proteins would be saturated soon after the beginning of microfiltration. The amount of proteins that deposited on membranes was proportional to the volume filtrated. The result also showed that the existence of aggregates in solution was the major factor that caused membrane fouling. Formation of aggregates in protein solutions was studied. It was shown that the pumping process was the major cause for the formation of aggregates. High temperature, high concentration of proteins, and a pH value near isoelectric point can accelerate the formation of aggregates. The results also showed that the amount of aggregates formed in solutions was linearly proportional to the average shear rate generated by pumping. Flux decline in microfiltration was studied, and a mathematical model describing flux was constructed. The model was in good agreement with the experimental data. Parameters of the model were directly related to the concentration of aggregates and the pumping treatment of the solution. The size of aggregates can be calculated with the values of the model parameters, and their size was similar to that reported in the literature.
语种	中文
公开日期	2013-09-16
页码	110
内容类型	学位论文
源URL	[http://ir.ipe.ac.cn/handle/122111/1373]
专题	过程工程研究所_研究所（批量导入）
推荐引用方式 GB/T 7714	张建东. 蛋白质溶液微滤过程中膜污染机理的研究[D]. 中国科学院过程工程研究所. 中国科学院过程工程研究所. 2003.

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