Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas

	Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas
	Zhang LinLin; Li Li; Zhang GuoFan
刊名	CHINESE SCIENCE BULLETIN
	2012-09-01
卷号	57 期号:25 页码:3312-3319
关键词	Crassostrea gigas FREPs fibrinogen-related proteins recombination oysters
ISSN号	1001-6538
通讯作者	Li, L (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
英文摘要	Fibrinogen-related proteins (FREPs) are lectins with at least a fibrinogen-like domain. They are widespread in Mollusca and play an important role as immune pattern-recognition receptors. These diverse molecules provide interesting models for the study of the evolution of innate immunity. Although oysters are among the most studied mollusks, there have been few investigations of this gene family in oysters. This paper reports the cloning of the first oyster FREP, named CgFREP, from the Pacific oyster Crassostrea gigas. Five types of FREP (CgFREP-1 to CgFREP-5) were obtained based on a single pair of primers, confirming their high diversity. The proteins deduced from these FREPs contained 253-363 amino acids and were divided into three subfamilies based on sequence identities (cutoff point < 86%). The topological structure of the phylogenetic tree showed the five CgFREPs clustered together and were completely separated from the subgroup of FREPs obtained from Biomphalaria glabrata (BgFREPs). This implies that FREP diversity evolved before the BgFREPs branched off. An interesting aspect of the CgFREPs was their individual hypermutation. Ninety-three SNPs and five indels were characterized among CgFREPs. Some mutations (e.g. an insertion in CgFREP-5) could cause premature translation termination. Multiple alignments suggest recombination events might have occurred during exchanges between alleles, or by somatic diversification, or both. Individual sequence variability parallels the extraordinary complexity of the innate immune system, suggesting a potential capacity to recognize and eliminate many kinds of pathogens, even in a primitive system. The diversity of immune pattern-recognition receptors may offer an alternative strategy that functions in the absence of highly specific immune globulins, which only emerged in vertebrates.
学科主题	Multidisciplinary Sciences
收录类别	SCI
原文出处	10.1007/s11434-012-5155-6
语种	英语
WOS记录号	WOS:000307750500009
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12245]
专题	海洋研究所_海洋生物技术研发中心
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang LinLin,Li Li,Zhang GuoFan. Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas[J]. CHINESE SCIENCE BULLETIN,2012,57(25):3312-3319.
APA	Zhang LinLin,Li Li,&Zhang GuoFan.(2012).Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas.CHINESE SCIENCE BULLETIN,57(25),3312-3319.
MLA	Zhang LinLin,et al."Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas".CHINESE SCIENCE BULLETIN 57.25(2012):3312-3319.