Molecular cloning and characterization of the myostatin gene in a cultivated variety of bay scallop, Argopecten irradians

	Molecular cloning and characterization of the myostatin gene in a cultivated variety of bay scallop, Argopecten irradians
	Guo, Lin1,2 ; Li, Li 1; Zhang, Shoudu 1,2; Zhang, Guofan 1
刊名	AQUACULTURE
	2012-06-20
卷号	350 页码:192-199
关键词	Myostatin Bay scallop Argopecten irradians "Zhongkehong"
ISSN号	0044-8486
通讯作者	Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	In this study, we cloned and characterized the myostatin (MSTN) gene from a cultivated strain of bay scallop, Argopecten irradians, "Zhongkehong", and named it as the Ai-MSTN gene. The Ai-MSTN cDNA sequence was 3553 bp, contained three exons with 1380 bp in the open reading frame encoding 459 amino acids, and was 2014 bp longer than one previously reported. Analysis of its genomic structure and expression profiles indicated that the sequence of MSTN was 12473 bp (Genbank: JN684902) containing 3 exons and 2 introns, which was conserved with its vertebrate and invertebrate homologs. In silico analysis revealed that the promoter region of Ai-MSTN contained several muscle specific regulatory elements. The 3D structure of Ai-MSTN was similar to that of the known mouse MSTN. Ai-MSTN was expressed not only in the digestive gland, pericardium, gill, adductor muscle, gonad and mantle of the bay scallop, but also in its hemolymph, which was firstly reported in mollusks. These findings provide genomic and protein information and expression profiles of the Ai-MSTN gene and lay the groundwork for further investigations of its functional significance in this economically important sea food, bay scallops. (C) 2012 Elsevier B.V. All rights reserved.
学科主题	Fisheries ; Marine & Freshwater Biology
收录类别	SCI
原文出处	10.1016/j.aquaculture.2012.03.040
语种	英语
WOS记录号	WOS:000305068800027
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12235]
专题	海洋研究所_海洋生物技术研发中心海洋研究所_文献信息中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Guo, Lin,Li, Li,Zhang, Shoudu,et al. Molecular cloning and characterization of the myostatin gene in a cultivated variety of bay scallop, Argopecten irradians[J]. AQUACULTURE,2012,350:192-199.
APA	Guo, Lin,Li, Li,Zhang, Shoudu,&Zhang, Guofan.(2012).Molecular cloning and characterization of the myostatin gene in a cultivated variety of bay scallop, Argopecten irradians.AQUACULTURE,350,192-199.
MLA	Guo, Lin,et al."Molecular cloning and characterization of the myostatin gene in a cultivated variety of bay scallop, Argopecten irradians".AQUACULTURE 350(2012):192-199.