Single nucleotide polymorphisms in i-type lysozyme gene and their correlation with vibrio-resistance and growth of clam Meretrix meretrix based on the selected resistance stocks

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	Single nucleotide polymorphisms in i-type lysozyme gene and their correlation with vibrio-resistance and growth of clam Meretrix meretrix based on the selected resistance stocks
	Yue, Xin 1; Wang, Hongxia 1; Huang, Xiaohong 1; Wang, Chao 1; Chai, Xueliang 2; Wang, Chunde 3; Liu, Baozhong1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012-09-01
卷号	33 期号:3 页码:559-568
关键词	Meretrix meretrix Vibrio-resistance Lysozyme SNPs
ISSN号	1050-4648
通讯作者	Liu, BZ (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	I-type lysozyme is considered to play crucial roles in both anti-bacteria and digestion function of the bivalve, which signifies that it is related to both immunity and growth. In this study, based on the principle of case-control association analysis, using the stock materials with different vibrio-resistance profile obtained by selective breeding, single nucleotide polymorphisms (SNPs) in the DNA partial sequence of an i-type lysozyme of Meretrix meretrix (MmeLys) were discovered and examined for their association with vibrio-resistance and growth. Twenty-seven SNPs were detected and fifteen of them were genotyped in clam stocks with different resistance to Vibrio harveyi (09-C and 09-R) and to Vibrio parahaemolyticus (11-S and 11-R). Allele frequency distribution among different stocks was compared. And wet weight of clams with different genotype at each SNP locus was compared. The results indicated that SNP locus 9 was associated with V. harveyi and V. parahaemolyticus resistance and growth of M. meretrix. Loci 12 and 14 were associated with both V. parahaemolyticus-resistance and growth, and also have the potential to be related with V harveyi-resistance of M. meretrix. Therefore these three SNPs especially locus 9 were the potential markers which may be involved in assisting resistance selective breeding. In addition, this study showed evidence that improvements in clam resistance to vibriosis could be achieved through selective breeding. All results provided encouragement for the continuation of the selective breeding program for vibrio-resistance gain in clam M. meretrix and the application of polymorphisms in MmeLys to the future marker assisted selection. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
原文出处	10.1016/j.fsi.2012.06.007
语种	英语
WOS记录号	WOS:000308057700011
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12384]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Zhejiang Mariculture Res Inst, Wenzhou 325005, Peoples R China 3.Qingdao Agr Univ, Qingdao 266109, Peoples R China
推荐引用方式 GB/T 7714	Yue, Xin,Wang, Hongxia,Huang, Xiaohong,et al. Single nucleotide polymorphisms in i-type lysozyme gene and their correlation with vibrio-resistance and growth of clam Meretrix meretrix based on the selected resistance stocks[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(3):559-568.
APA	Yue, Xin.,Wang, Hongxia.,Huang, Xiaohong.,Wang, Chao.,Chai, Xueliang.,...&Liu, Baozhong.(2012).Single nucleotide polymorphisms in i-type lysozyme gene and their correlation with vibrio-resistance and growth of clam Meretrix meretrix based on the selected resistance stocks.FISH & SHELLFISH IMMUNOLOGY,33(3),559-568.
MLA	Yue, Xin,et al."Single nucleotide polymorphisms in i-type lysozyme gene and their correlation with vibrio-resistance and growth of clam Meretrix meretrix based on the selected resistance stocks".FISH & SHELLFISH IMMUNOLOGY 33.3(2012):559-568.